April 1965
Volume 4, Issue 2
Free
Articles  |   April 1965
A Study of the DNA of Dogfish Corneal Epithelium
Author Affiliations
  • SEYMOUR ZIGMAN
    Departments of Ophthalmology and Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, N. Y.
  • GEORGE MUNRO
    Departments of Ophthalmology and Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, N. Y.
  • SIDNEY LERMAN
    Departments of Ophthalmology and Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, N. Y.
Investigative Ophthalmology & Visual Science April 1965, Vol.4, 222-225. doi:
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      SEYMOUR ZIGMAN, GEORGE MUNRO, SIDNEY LERMAN; A Study of the DNA of Dogfish Corneal Epithelium. Invest. Ophthalmol. Vis. Sci. 1965;4(2):222-225.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Small Sephadex G-200 columns were employed to purify milligram quantities of DNA extracted from dogfish cornea epithelium with sodium dodecyl sulfate, and studies of the composition and physical properties were made. 3H thymidine and 125I deoxyuridine were incorporated into epithelial DNA under simple in vitro circumstances. The incorporation of thymidine depended upon the temperature and oxygen tension of the incubation medium.

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