April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Inhibitory effect of tranilast on transforming growth factor betaexpression in corneal fibroblasts derived from granular corneal dystrophy type II
Author Affiliations & Notes
  • Ae Young Kwak
    The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
    Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Hun Lee
    The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
    Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Kyoung Yul Seo
    The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
    Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Hyung Keun Lee
    The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
    Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Eung Kweon Kim
    The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
    Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Tae-im Kim
    The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea
    Cornea Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Ae Young Kwak, None; Hun Lee, None; Kyoung Yul Seo, None; Hyung Keun Lee, None; Eung Kweon Kim, Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education(351-2009-2-E00061) (F), Converging Research Center Program funded by the Ministry of Education, Science and Technology (2012K001354) (F), Grant MEST (2009-0066392) from the National Research Foundation of Korea (F); Tae-im Kim, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1008. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ae Young Kwak, Hun Lee, Kyoung Yul Seo, Hyung Keun Lee, Eung Kweon Kim, Tae-im Kim; Inhibitory effect of tranilast on transforming growth factor betaexpression in corneal fibroblasts derived from granular corneal dystrophy type II. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1008.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To investigate the effects of tranilast on expression of transforminggrowth factor beta-induced protein (TGFBIp) in wild-type (WT) and homozygous (HO) granular corneal dystrophy type II (GCD 2) corneal fibroblasts

Methods: Corneal fibroblasts were incubated with tranilast (0.01, 0.025, 0.05,0.1, 0.25, 0.5 or 1.0 mM), after which cell proliferation and cytotoxicity were measured. Changes in expression of TGFBIp and TGFBI mRNA after application of tranilast in WT and HO GCD2 corneal fibroblasts co-treated with TGF-β (5.0 ng/mL) were examined by Western blot analysis and real-time polymerase chain reaction, respectively. Furthermore, effects of tranilast on type I collagen, phosphorylated Smad2 (pSmad2) and pSmad3 expression, wound healing assay of corneal fibroblasts, alpha-smooth muscle actin (α-SMA) expression, and integrin expression were evaluated.

Results: Tranilast decreased the number of viable corneal fibroblasts, but didnot induce cytotoxicity. TGF-β increased expression of TGFBIp and TGFBI mRNA in WT and HO GCD2 corneal fibroblasts. However, application of tranilast in corneal fibroblasts co-treated with TGF-β reduced levels of TGFBIp and TGFBI mRNA. TGF-β increased expression of type I collagen and pSmad2 in WT and HO GCD2 corneal fibroblasts, but pSmad3 in HO GCD2 corneal fibroblasts. Application of tranilast reduced levels of type I collagen and pSmad2 in WT and HO corneal fibroblasts co-treated with TGF-β, and pSmad3 in HO corneal fibroblasts. Tranilast delayed wound healing by inhibiting expression of α-SMA and integrins.

Conclusions: Tranilast can be used for delaying or prevention of recurrence of corneal opacity in TGFBI-linked corneal dystrophies by inhibiting TGF-β signaling pathway.

Keywords: 480 cornea: basic science • 533 gene/expression • 739 transcription factors  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×