April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Th1-, 2-, and Th17-relevant cytokine levels in vitreous humor of sarcoidosis patients with uveitis
Author Affiliations & Notes
  • masaki shibata
    national defense medical collage, Saitama, Japan
  • Tomohito Sato
    national defense medical collage, Saitama, Japan
  • Atsushi Tanaka
    national defense medical collage, Saitama, Japan
  • Yoko Karasawa
    national defense medical collage, Saitama, Japan
  • Manzo Taguchi
    national defense medical collage, Saitama, Japan
  • Tadashi Muraoka
    national defense medical collage, Saitama, Japan
  • Masataka Ito
    national defense medical collage, Saitama, Japan
  • Masaru Takeuchi
    national defense medical collage, Saitama, Japan
  • Footnotes
    Commercial Relationships masaki shibata, None; Tomohito Sato, None; Atsushi Tanaka, None; Yoko Karasawa, None; Manzo Taguchi, None; Tadashi Muraoka, None; Masataka Ito, None; Masaru Takeuchi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 101. doi:
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      masaki shibata, Tomohito Sato, Atsushi Tanaka, Yoko Karasawa, Manzo Taguchi, Tadashi Muraoka, Masataka Ito, Masaru Takeuchi; Th1-, 2-, and Th17-relevant cytokine levels in vitreous humor of sarcoidosis patients with uveitis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):101.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Elevated levels of cellular mediators, including cytokines, chemokines, and growth factors are frequently observed in vitreous humor of patients with uveitis. The study was conducted to investigate involvement of Th17-mediated immune responses in granulomatous uveitis associated with sarcoidosis by analyzing the cytokine levels in the vitreous.

Methods: Levels of 15 cytokines, IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, soluble CD40L, IFN-γ, and TNFα were measured: by using multiplex bead analysis of vitreous humor of 11 eyes (8 patients) with sarcoidosis undergoing vitrectomy for a variety of vitreoretinal complications refractory for medical treatments between August 2012 and November 2013. These vitreous cytokine levels were compared with 9 eyes (9 patients) with epiretinal membrane and 7 eyes (7 patients) with macular hole (MH). Statistical differences were analyzed by ANOVA’s test.

Results: IL-4, IL-6, IL-17A, IL-21, IL-22, IL-31, IFN-g, SCD40L, and TNFa were detected in the vitreous humor of eyes with sarcoidosis (, and , respectively), ERM (, and , respectively), and MH (, and , respectively), in which vitreous levels of IL-4, IL-6, IL-17A, IL-21, IL-25, IL-31, IFN-γ, and sCD40L were more in eyes with sarcoidosis than the other. However, vitreous levels significantly elevated in eyes with sarcoidosis was only IFN-γ.

Conclusions: These results indicated that Th1-mediated immune responses were more critical than Th17 responses for development of uveitis in sarcoidosis patients. Alternatively, it is also possible that activation of Th17 cells was more susceptible than Th1 cells for medical treatment with corticosteroid to suppress ocular inflammation before surgery.

Keywords: 746 uveitis-clinical/animal model • 490 cytokines/chemokines • 554 immunohistochemistry  
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