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Qianli Meng, Mengyuan Fang, Haike Guo, Liya Wang, Liang Zhang, Ying Cui; PD-1 involved in the development of proliferative diabetic retinopathy by mediating activated-induced apoptosis rather than Th1 and Th2 response. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1032.
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© ARVO (1962-2015); The Authors (2016-present)
To study the expression and functional characteristics of programmed death-1 (PD-1) pathway in peripheral blood lymphocytes of patients with proliferative diabetic retinopathy (PDR).
Peripheral blood lymphocytes were obtained from patients with PDR, age-matched diabetes mellitus with no diabetic retinopathy (DM-NDR) and controls. The mRNA expression of PD-1 and its ligands was observed by real-time polymerase chain reaction. Flow cytometric assay was used to determine the protein expression of PD-1 and its ligands, the frequencies of activation-induced apoptosis, IFN-γ and IL-4. The relationship between β2-microglobulin and these exploratory variables was analyzed.
The mRNA expression of PD-1 was significantly lower while its protein expression was markedly increased in PDR group compared with DM-NDR and control groups. The mRNA and protein expression of PD-L1 in PDR group was lower than in the other groups. In PDR group, there was a higher expression of activation-induced apoptotic lymphocytes, and the frequency of Annexin V+PI-PD-1+ cells were increased while the frequency of Annexin V+PI-PD-L1+ cells was decreased. Although the expression of PD-1+ IFN-γ+ and PD-1+IL-4+ cells in PDR group was upregualted, their ration was no significantly differences compared with DM-NDR and control groups. β2-microglobulin from patients with PDR positively correlated with PD-1, activation-induced apoptosis and IFN-γ, while it negatively correlated with IL-4.
PD-1 is involved in the development of PDR by mediating activated-induced apoptosis rather than Th1 and Th2 response. The positive relationship with PD-1 and β2-microglobulin suggests that PD-1 may be used to predict the diabetic microangiopathy.
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