April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Epigenetic changes in melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells
Author Affiliations & Notes
  • Tingting Jiang
    Eye and ENT Hospital, Fudan University,, Shanghai, China
  • Qing Chang
    Eye and ENT Hospital, Fudan University,, Shanghai, China
  • Gezhi Xu
    Eye and ENT Hospital, Fudan University,, Shanghai, China
  • Footnotes
    Commercial Relationships Tingting Jiang, None; Qing Chang, None; Gezhi Xu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1054. doi:
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      Tingting Jiang, Qing Chang, Gezhi Xu; Epigenetic changes in melatonin-Mediated Cytoprotection against Hyperglycemic Injury in Müller Cells. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1054.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Inflammation plays important roles in the development of diabetic retinopathy (DR). The aim of this study was to investigate the epigenetic modulation by melatonin to attenuate inflammatory response in Müller cells under hyperglycemic condition.

 
Methods
 

Primary cultured Müller cells were divided into several groups, treated with either high glucose medium (containing 30 mM glucose) alone or combined with melatonin (0.1 mM) for various time. Melatonin was dissolved in dimethylsulfoxide (DMSO) and the stock solutions were added directly to the serum-free culture media. In the osmotic control group, cells were treated with medium containing 5.5 mM glucose and 24.5 mM mannitol. TNFα, IL1βexpressions were measured by Real-time PCR. HDAC4, HDAC5, AcH3K9 and AcH4K5mRNA and protein expressions were measured by Real-time PCR and Western Blot. Data were analyzed with the SPSS 16 software (IBM). Means ± SD were calculated for each group. For multiple comparisons, one-way ANOVA followed by Tukey's multiple comparison tests were used. Differences were considered statistically significant when p-value was less than 0.05.

 
Results
 

TNFαand IL1βexpressions were upregulated by high glucose stimulation compared with the control group (P<0.05) and melatonin attenuated the expressions (P<0.05). Under hyperglycemic condition, the levels of HDAC4 and HDAC5 were reduced, while AcH3K9 and AcH4K5 levels were elevated (P<0.05). However, melatonin reversed the effects. (P<0.05)

 
Conclusions
 

These findings suggest epigenetic regulation by melatonin may contribute to its anti-inflammatory effect on DR.

     
Keywords: 499 diabetic retinopathy  
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