April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
The G-Protein Coupled Receptor P2Y2 is Upregulated in the Diabetic Rat Retina. Possible Role in Diabetic Retinopathy.
Author Affiliations & Notes
  • Juan E Gallo
    Nanomedicine & Vision Group - Facultad de Ciencias Biomedicas, Universidad Austral, Pilar, Argentina
    Ophthalmology, Hospital Universitario Austral, Pilar, Argentina
  • Jorge Mancini
    Nanomedicine & Vision Group - Facultad de Ciencias Biomedicas, Universidad Austral, Pilar, Argentina
    Ophthalmology, Hospital Universitario Austral, Pilar, Argentina
  • Gustavo Ortiz
    Nanomedicine & Vision Group - Facultad de Ciencias Biomedicas, Universidad Austral, Pilar, Argentina
  • Juan Croxatto
    Ocular Pathology, Fundación Oftalmologica Argentina "Jorge Malbran", Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships Juan Gallo, None; Jorge Mancini, None; Gustavo Ortiz, None; Juan Croxatto, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1057. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Juan E Gallo, Jorge Mancini, Gustavo Ortiz, Juan Croxatto, ; The G-Protein Coupled Receptor P2Y2 is Upregulated in the Diabetic Rat Retina. Possible Role in Diabetic Retinopathy.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1057.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Purinergic P2Y2r have been reported to be involved in inflammation and to have a neuroprotective effect. We aimed at evaluating the role of P2Y2r in experimental diabetic retinopathy

Methods: Three groups of 5 male Wistar rats with a weight of 250g were treated with an intraperitoneal injection of 45mg/kg of streptozotocin. Animals with glycemia levels above 200 mg/dl were included in the study. A control group was made up of 5 non-diabetic rats. PPADS and suramin, purinergic antagonists, were twice intraperitoneally injected to a group of diabetic animals each, at 9 and 24 weeks of diabetes. Animals were sacrificed at 28 weeks of diabetes. Retinas were analyzed by immunohistochemistry and western blot using primary antibodies against P2Y2r, GFAP, VEGF-A and Caspase-3.

Results: P2Y2r immunoreactivity was found in the fiber layer of non-diabetic and diabetic animals. These animals also showed immunostaining of P2Y2r at the photoreceptor outer segment. Western-blot confirmed the higher expression of P2Y2r in diabetics than controls. Diabetic animals treated with PPADS showed a higher protein expression of P2Y2r than those without treatment. Besides, PPADS and Suramin diabetic groups had higher protein expressions of VEGF-A and Caspase-3 than that found in diabetic animals without therapy.

Conclusions: P2Y2r seems to be involved in inflammatory and apoptotic mechanisms in experimental diabetic retinopathy. Results suggest that P2Y2r might have a protective effect in the diabetic rat retina

Keywords: 499 diabetic retinopathy • 557 inflammation • 675 receptors: pharmacology/physiology  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×