April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effects of COMP-Ang1 on Neovascularization in a Mouse Model of Age-related Macular Degeneration
Author Affiliations & Notes
  • Nathan Lambert
    University of Utah, Salt Lake City, UT
  • Xiaohui Zhang
    University of Utah, Salt Lake City, UT
  • Judd Cahoon
    University of Utah, Salt Lake City, UT
  • Hironori Uehara
    University of Utah, Salt Lake City, UT
  • Balamurali Ambati
    University of Utah, Salt Lake City, UT
  • Footnotes
    Commercial Relationships Nathan Lambert, Fight For Sight (F); Xiaohui Zhang, None; Judd Cahoon, None; Hironori Uehara, None; Balamurali Ambati, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1187. doi:
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      Nathan Lambert, Xiaohui Zhang, Judd Cahoon, Hironori Uehara, Balamurali Ambati; Effects of COMP-Ang1 on Neovascularization in a Mouse Model of Age-related Macular Degeneration. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1187.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To determine the effect of COMP-Ang1 on vessel proliferation and VEGF levels in a mouse model of AMD

 
Methods
 

C57Bl6 mice received 1µL subretinal injections of PBS or a viral vector (AAV2) expressing LacZ, GFP, or COMP-Ang1. 1 month later mice underwent laser-induced CNV (532-nm diode laser spots, 140mW, 100msec, 50µm). Laser lesions were induced at locations of 3, 6, 9, and 12 o’clock 2-3 disc diameters from the optic nerve in each eye. One week after laser CNV, mice eyes were enucleated, the choroid was stained for CD31 markers using immunohistochemistry, and then prepared as choroidal flatmounts. CNV volume was assessed using confocal microscopy and quantified using Image J software.

 
Results
 

CNV volume was significantly decreased in mice with AAV2.COMP-Ang1 treatment compared with AAV2.GFP, AAV2.LacZ, and PBS groups. The CNV volume for the various groups was as follows: +AAV2.COMP-Ang1 6.36 ± 1.94 X 104 µm3 (n=31)* +AAV2.LacZ 15.75 ± 4.91 X 104 µm3 (n=38) +AAV2.GFP 21.32 ± 4.57 X 104 µm3 (n=30) +PBS 16.12 ± 3.155 X 104 µm3 (n=40) COMP-Ang1 decreased laser CNV volume nearly 2.5x when compared to PBS (P=0.017) and AAV2.LacZ (P=0.1) and over 3.3x compared to AAV2.GFP (P=0.003).

 
Conclusions
 

These findings provide evidence that AAV2 expression of COMP-Ang1 decreases laser CNV volume in a mouse model. Some limitations to this study include size and shape of laser-induced CNV growth. Further studies will address the effects COMP-Ang1 on VEGF levels.

  
Keywords: 412 age-related macular degeneration  
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