April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Immunohistochemical observation of meibomian gland following ocular surface alkali burning in wild-type and Smad3-deficient mice.
Author Affiliations & Notes
  • Shin Mizoguchi
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Yuka Okada
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Reiko Arita
    Ophthalmology, Itoh Clinic, Saitama, Japan
  • Shizuya Saika
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships Shin Mizoguchi, None; Yuka Okada, None; Reiko Arita, None; Shizuya Saika, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 13. doi:
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      Shin Mizoguchi, Yuka Okada, Reiko Arita, Shizuya Saika; Immunohistochemical observation of meibomian gland following ocular surface alkali burning in wild-type and Smad3-deficient mice.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):13.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To immunohistochmeically examine the pathology of meibomian glands following ocular surface alkali burning and investigate the effects of lacking Smad3 in alkali burn-induced alteration of meibomian glands.

Methods: Three μL of 1 N NaOH were applied under general anesthesia to the right eye of 6 - 8 week old wild-type (n = 5) and Smad3 (n = 10) mice to produce a total ocular surface alkali burn. Mice were killed and eyelid was processed for histology of meibomian glands at day 5, 10 and 20 post-treatment. Hematoxilin-Eosin, oil red O staini and immunohistochemistory for α-smooth muscle actin were employed.

Results: Histology showed extremely dilated lumen of the meibomian glands in alkali-burned tissue. The dilated lumen was filled with Oil red O-labeled meibum. Different from uninjured mebomian glands, dilated lumen was rapped with a multiulayer of flattened cells that was labeled with anti anti-αSMAantibody. Lacking Smad3 did not affect the pathology of the treated meibomian glands.

Conclusions: Meibomian glnad cells underwent epithelial-mesenchymal transition following ocular surface alkali exposure. Obstruction of the gland orifice might accumulate meibum, leading extreme dilation of the gland lumen. Smad3 signal might not be involved in EMT of meibomian gland cells in the pathological condition.

Keywords: 526 eyelid • 486 cornea: tears/tear film/dry eye • 512 EMT (epithelial mesenchymal transition)  
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