April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Subpopulations of human umbilical cord mesenchymal stem cells exhibit differential rescusing functions on retinal degeneration in RCS rats
Author Affiliations & Notes
  • Li Wang
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Haibin Tian
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Peng Li
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Zongyi Li
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Chunpin Lian
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Qingjian Ou
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Lixia Lu
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Weiye Li
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Ophthalmology, Drexel University College of Medicine, Philadelphia, PA
  • Guotong Xu
    Department of Ophthalmology of Shanghai Tenth Hospital, and Tongji Eye institute, Tongji University School of Medicine, Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine, Shanghai, China
  • Footnotes
    Commercial Relationships Li Wang, None; Haibin Tian, None; Peng Li, None; Zongyi Li, None; Chunpin Lian, None; Qingjian Ou, None; Lixia Lu, None; Weiye Li, None; Guotong Xu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1382. doi:
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      Li Wang, Haibin Tian, Peng Li, Zongyi Li, Chunpin Lian, Qingjian Ou, Lixia Lu, Weiye Li, Guotong Xu; Subpopulations of human umbilical cord mesenchymal stem cells exhibit differential rescusing functions on retinal degeneration in RCS rats. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1382.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To provides more information about the heterogeneity of human umbilical cord mesenchymal stem cells (UCMSCs) and a new strategy for UCMSCs treatment of retinal degeneration (RD) by choosing appropriate subpopulations.

Methods: Two subsets of human umbilical cord mesenchymal stem cells (UCMSCs), termed as UCMSC1 and UCMSC2, were isolated by single cell cloning, specific surface markers were analyzed by flow cytometry. Proliferating rate and gene expression were carried out by MTT and RNA sequencing analysis. The gene levels of growth factors and cytokines were measured by Real-time PCR. Their differentiation abilities were confirmed by culturing UCMSC in adipogenic, osteogenic and chondrogenic differentiation media. UCMSC1 and UCMSC2 were transplanted into subretinal space of RCS rats, their therapeutic functions were confirmed by analyzing retinal nuclear layer thickness, apoptotic photoreceptors and electroretinogram.

Results: Both the two subpopulations share similar membrane marker phenotypes. However, UCMSC1 showed stronger ability than UCMSC2 regarding proliferating rate, colony forming ability, adipogenic and osteogenic potential, whereas the latter exhibited increased chondrogenic ability. RNA sequencing analysis further showed the differential gene levels relative to proliferation, differentiation and immunoregulation in UCMSC1 and UCMSC2. After transplanted into subretinal space of RCS rats, UCMSC1 had stronger vision rescue function compared to UCMSC2, including increased b¬-wave amplitude, retinal nuclear layer thickness, and decreased apoptotic photoreceptors. Furthermore, When subjected to interleukin-6 treatment in vitro, mimicking the transplanted MSCs under inflammation condition in RD, UCMSC1 expressed much higher levels of growth factors than UCMSC2, such as bFGF, CNTF, EGF, HGF, PEDF, which indicates that the differential therapeutic capacities of UCMSC subpopulations involve distinct paracrine functions.

Conclusions: Two subpopulations with distinct morphology, proliferation, differentiation potentials, marker expression and gene expression were derived from human UCMSCs. In addition, the different subpopulations of human UCMSCs had distinct therapeutic functions on RD.

Keywords: 721 stem cells • 412 age-related macular degeneration • 695 retinal degenerations: cell biology  
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