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Jacqueline Faustino, Davi Marques, Ana Carolina Dias, Monica Alves, Carolina Maria Modulo, Adriana Murashima, Lilian Eslaine, Peter S Reinach, Eduardo M Rocha; Tear film and ocular surface changes in rat models of ocular toxicity and dry eye. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1497.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the tear film, systemic and ocular surface parameters in three animal models of dry eye syndrome(DES):a) induced by benzalconium chloride0.2% (BAC)instillation, BID for 7 days; b) Diabetes Mellitus (DM) induced by streptozotocin (60mg/kgiv)and c) Hypothyroidism (HT) induced by methimazole (500mg/l of drinking water) after 5 weeks.
Male rats treated as described above were compared to controls (n=5-8/group). The tear film and blood osmolarity were measured. Phenol red thread tear test and fluorescein staining were performed and compared among the groups. Cornea histology was evaluated after H&E staining.
Tear flow was significantly lower in the three groups compared to controls rats (p=0.0062).Fluorescein staining was significantly higher in the BAC group (p=0.01). In controls,the tear omolarity was 284.0±3.29 mOsm/l,in BAC it was 306 ±4.09 mOsm/l, in DM it was 337.8±17.35 mOsm/l and in HT it was 338.0±13.5 mOsm/l (p=0.0037).Blood osmolarity in DM group was 328.4±1.33mOsm/l, in the control group it was 302.2± 6.35 mOsm/l and in HT group it was 339±30.2mOsm/land BAC group 321±2.82 (p=0.0058).Cornea epithelium was thinner at day 07 in the BAC group (22.9±3.6 μm) and DM (22.2±5.8 μm)but not in HT group(31.8±3.0 μm)compared with control rats (29.2±1.7 μm) (p=0.0055).
The ocular surface changes and hyperosmolarity in animal models resembled that of DES in humans.Moreover, the present work suggests that systemic hyperosmolarity may be involved in the mechanism of some conditions related to DES.
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