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Ithar M Beshtawi, Riaz Akhtar, Chantal Hillarby, Clare O, Xuegen Zhao, Arun Brahma, Fiona Carley, Brian Derby, Hema Radhakrishnan, ; Biomechanical Stiffness Changes of Collagen Crosslinking on Human Keratoconic Corneas using Scanning Acoustic Microscopy. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1550.
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© ARVO (1962-2015); The Authors (2016-present)
To assess the biomechanical stiffness of collagen cross-linking on keratoconic corneas, in-vitro
Six keratoconic corneal buttons were included in this study. Each cornea was divided into two halves, where one half was cross-linked and the other half was treated with riboflavin only and served as control. The stiffness of the corneal tissue was measured across the stroma using scanning acoustic microscopy (SAM).
In the cross-linked corneas, there was a steady decrease in the magnitude of speed of sound from the anterior region through to the posterior regions of the stroma. The speed of sound was found to remain relatively unchanged in the control corneas. The increase in stiffness between the cross-linked and control corneas in the anterior 200 × 200 μm region was by a factor of 1.039x.
A higher speed of sound was detected in cross-linked keratoconic corneal tissue when compared with their controls, using SAM. This in vitro model can be used to compare to the cross-linking results obtained in-vivo, as well as comparing the results obtained with different protocols.
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