April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Biomechanical Stiffness Changes of Collagen Crosslinking on Human Keratoconic Corneas using Scanning Acoustic Microscopy
Author Affiliations & Notes
  • Ithar M Beshtawi
    An-Najah National University, Nablus, Palestine, State of
  • Riaz Akhtar
    University of Liverpool,, Liverpool, United Kingdom
  • Chantal Hillarby
    The University of Manchester, Manchester, United Kingdom
  • Clare O
    The University of Manchester, Manchester, United Kingdom
  • Xuegen Zhao
    The University of Manchester, Manchester, United Kingdom
  • Arun Brahma
    Manchester Royal Eye Hospital, Manchester, United Kingdom
  • Fiona Carley
    Manchester Royal Eye Hospital, Manchester, United Kingdom
  • Brian Derby
    The University of Manchester, Manchester, United Kingdom
  • Hema Radhakrishnan
    The University of Manchester, Manchester, United Kingdom
  • Footnotes
    Commercial Relationships Ithar Beshtawi, None; Riaz Akhtar, None; Chantal Hillarby, None; Clare O, None; Xuegen Zhao, None; Arun Brahma, None; Fiona Carley, None; Brian Derby, None; Hema Radhakrishnan, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 1550. doi:
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      Ithar M Beshtawi, Riaz Akhtar, Chantal Hillarby, Clare O, Xuegen Zhao, Arun Brahma, Fiona Carley, Brian Derby, Hema Radhakrishnan, ; Biomechanical Stiffness Changes of Collagen Crosslinking on Human Keratoconic Corneas using Scanning Acoustic Microscopy. Invest. Ophthalmol. Vis. Sci. 2014;55(13):1550.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To assess the biomechanical stiffness of collagen cross-linking on keratoconic corneas, in-vitro

 
Methods
 

Six keratoconic corneal buttons were included in this study. Each cornea was divided into two halves, where one half was cross-linked and the other half was treated with riboflavin only and served as control. The stiffness of the corneal tissue was measured across the stroma using scanning acoustic microscopy (SAM).

 
Results
 

In the cross-linked corneas, there was a steady decrease in the magnitude of speed of sound from the anterior region through to the posterior regions of the stroma. The speed of sound was found to remain relatively unchanged in the control corneas. The increase in stiffness between the cross-linked and control corneas in the anterior 200 × 200 μm region was by a factor of 1.039x.

 
Conclusions
 

A higher speed of sound was detected in cross-linked keratoconic corneal tissue when compared with their controls, using SAM. This in vitro model can be used to compare to the cross-linking results obtained in-vivo, as well as comparing the results obtained with different protocols.

     
Keywords: 480 cornea: basic science  
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