April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Development of Surgical Glaucoma Model in the Miniature Swine
Author Affiliations & Notes
  • Alain Stricker-Krongrad
    Sinclair Research Center LLC, Auxvasse, MO
  • Sarah Renna
    Sinclair Research Center LLC, Auxvasse, MO
  • Cecil P Moore
    Sinclair Research Center LLC, Auxvasse, MO
  • Jason Liu
    Sinclair Research Center LLC, Auxvasse, MO
  • Brian Hanks
    Sinclair Research Center LLC, Auxvasse, MO
  • David White
    Sinclair Research Center LLC, Auxvasse, MO
  • Larry Brown
    Sinclair Research Center LLC, Auxvasse, MO
  • Guy Bouchard
    Sinclair Research Center LLC, Auxvasse, MO
  • Footnotes
    Commercial Relationships Alain Stricker-Krongrad, None; Sarah Renna, None; Cecil Moore, None; Jason Liu, None; Brian Hanks, None; David White, None; Larry Brown, None; Guy Bouchard, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2409. doi:
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      Alain Stricker-Krongrad, Sarah Renna, Cecil P Moore, Jason Liu, Brian Hanks, David White, Larry Brown, Guy Bouchard, ; Development of Surgical Glaucoma Model in the Miniature Swine. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2409.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The purpose of this project was to develop a model of glaucoma via surgical induction of increased intraocular pressure (IOP) in Yucatan miniature swine.

Methods: Three pigs (two female, one male) had bilateral IOP measurements performed prior to surgical intervention to establish a baseline IOP for each animal from which future changes in IOP could be identified. IOP measurements (mm Hg) were taken with a Tonopen Vet Tonometer. In order to reduce venous drainage from the eyes, episcleral veins were scarified by cauterization in each eye. IOPs were periodically measured for several weeks post-cauterization surgery. Pharmacologic intervention was then instituted with a commercially available synthetic prostamide analog with ocular hypotensive activity. Drops were applied once daily, and IOPs continued to be measured. After 7 weeks of daily treatment, eye drops were discontinued, and IOP measurements continued to be obtained.

Results: All animals presented with significant increases in IOP measurements post surgical intervention and significant decreases in IOP with pharmacological therapy. Statistical tests included sample mean comparison by 2-tail T-test P-value (T≤t). Data by phase as follows-Mean±SD (N readings), P-value: Baseline:19±4 (65); Post-Surgery: 24±5 (124), ≤0.005 (Baseline vs. Post-Surg); Treatment: 18±4 (75), ≤0.0006 (Post-Surg vs Treatment) Recovery: 20±4 (80), ≤0.006 (Treatment vs Recovery).

Conclusions: Therefore, the Yucatan miniature swine should be considered a viable model for surgically induced glaucoma. The miniature swine eye is also responsive to pharmacological therapy to reduce IOP and as such could be a potential model for future pharmacological research.

Keywords: 568 intraocular pressure • 420 anterior chamber • 749 vascular occlusion/vascular occlusive disease  
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