April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
C1q ablation protects Retinal Ganglion Cells in glaucoma.
Author Affiliations & Notes
  • Ruma Kumari
    SUNY Downstate Medical Center, Brooklyn, NY
  • Alina Genis
    SUNY Downstate Medical Center, Brooklyn, NY
  • Konstantin Astafurov
    SUNY Downstate Medical Center, Brooklyn, NY
  • Hai M Tang
    SUNY Downstate Medical Center, Brooklyn, NY
  • John Danias
    SUNY Downstate Medical Center, Brooklyn, NY
  • Footnotes
    Commercial Relationships Ruma Kumari, None; Alina Genis, None; Konstantin Astafurov, None; Hai Tang, None; John Danias, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 2432. doi:
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      Ruma Kumari, Alina Genis, Konstantin Astafurov, Hai M Tang, John Danias; C1q ablation protects Retinal Ganglion Cells in glaucoma.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2432.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To confirm that C1q ablation has a protective effect on Retinal Ganglion Cells (RGC) in glaucoma.

Methods: Congenic C1q Knockout (KO) mice were generated in the DBA2/NNia strain. Female animals with no C1q (KO), Heterozygous (HT) for C1q deletion and Wild type (WT) were aged. Retinas of mice (n=47 for KO; n=66 for HT; n=41 for WT) from all three groups at the ages of 6, 9 and 12 months were flat mounted after retrograde labeling, imaged and scored semi quantitatively on a 10 point scale by two independent observers. The total area of each flatmounted retina was also measured. One way analysis of variance (ANOVA) was used for comparisons between different groups with Bonferroni’s post-hoc testing.

Results: Retinal scores did not differ between the three groups of animals at 6 months of age (ANOVA, P>0.05). Retinal score differences of animals at 9 months of age also did not reach statistical significance (ANOVA, P>0.05). At 12 months of age C1q KO had higher retinal scores indicating more RGC preservation (P=0.02, P<0.05 post-hoc testing for comparison between WT & KO groups). No differences were detected in retinal areas at 6, 9 or 12 months of age between groups (ANOVA, p>0.05; P>0.05; P>0.05 respectively).

Conclusions: 1. Absence of C1q ameliorates RGC loss in DBA2/NNia strain and the C1q effect appears to be gene dose dependent. 2. Our results reinforce earlier findings that absence of C1q is protective in DBA2 mouse model of glaucoma.

Keywords: 695 retinal degenerations: cell biology  
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