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Daniela Garcia Romero, Alejandro Babayan, Oscar Fernandez, Karen Tellez-Alcaide, Atzin Robles-Contreras, Hector Javier Perez-Cano, Hospital de la luz; Polymorphism -1486T/C TLR 9 gene as a risk factor in infectious blepharitis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):2749.
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To demonstrate the association between polymorphism of -1486T/C TLR9 gene promoter region with infectious blepharitis.
Thirty samples of palpebral scraping from patients with infectious blepharitis, and thirty samples from not infected subjects, as controls, were studied. The promoter region of toll like receptor 9 (TLR 9) gene was amplified by PCR, and then, the PCR products were purified and analyzed the presence of SNP -1486T/C by direct sequencing. Fisher Chi-square was used to found the association between blepharitis and the polymorphism in the promoter region.
The allelic frequency of -1486T was 62.5% of the promoter region of TLR 9 gene in patients, and in controls was 25%. Our results showed significantly differences between patients and control subjects (p<0.0001) and the relative risk was 2.48 and the Odds Ratio 4.89.
In this study of Mexican population on infectious blepharitis and polymorphism of the promoter region of TLR 9 gene, we found high frequency for -1486T allele in patients with infectious blepharitis,which indicates that there is relationship between -1486T polymorphism in TLR 9 gene and is associated with the susceptibility for infectious blepharitis in the studied population.
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