April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Role of HMG protein in Primary Retinoblastoma
Author Affiliations & Notes
  • Mithalesh Singh
    Ocular Pathology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Seema Kashyap
    Ocular Pathology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Lata Singh
    Ocular Pathology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Neelam Pushker
    Ophthalmology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Seema Sen
    Ocular Pathology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Anjana Sharma
    Ocular Microbiology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Bhavna Chawla
    Ophthalmology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical sciences, New Delhi, India
  • Footnotes
    Commercial Relationships Mithalesh Singh, None; Seema Kashyap, None; Lata Singh, None; Neelam Pushker, None; Seema Sen, None; Anjana Sharma, None; Bhavna Chawla, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3075. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Mithalesh Singh, Seema Kashyap, Lata Singh, Neelam Pushker, Seema Sen, Anjana Sharma, Bhavna Chawla, ; Role of HMG protein in Primary Retinoblastoma. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3075.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Retinoblastoma is a malignant tumor composed of embryonic tumor cells from retinoblasts of neuroepithelial origin. High mobility group proteins (HMG) are the member of non histone nuclear factors associated with cell proliferation, differentiation and neoplastic transformation. High mobility group (HMG) proteins is a newly recognized protein regulating cancer cell tumorigenesis, expansion and invasion. However, the role of HMGB1 is still unclear in retinoblastoma.

Methods: Prospective analysis of 70 primary enucleated retinoblastoma cases over a period of one year. Expression of HMGB1 was performed by immunohistochemistry (IHC) in formalin fixed retinoblastoma specimens and their results were confirmed by western blotting. mRNA expression was performed by semi-quantitative Reverse Transcriptase PCR (RT-PCR).

Results: A total of 70 eyes were taken of which 10(14.28%) eyes had bilateral involvement. Ages ranged from 7months to 8years. 56 (80%) cases were reported as poorly differentiated tumors whereas 46(65.71%) and 14(20%) cases had calcification and necrosis respectively. Histopathologically, 16(22.85%) had massive choroid invasion, 13(18.57%) had optic nerve invasion, 6 cases each had scleral and ciliary body invasion. Strong expression of HMGB1 were seen in 41/70(58.57%) cases. mRNA expression was seen in 36 cases (51.4%) by RT-PCR. Expression of HMGB1 was statistically significant with poor differentiation (p=0.0436) and optic nerve invasion (p=0.0473).

Conclusions: Overexpression of HMGB1 is seen more in poorly differentiated tumors and those with, histopathological high risk factors. HMGB1 could serve as a poor prognostic marker in retinoblastoma. Better understanding of the molecular mechanisms underlying HMGB1 function could yield novel therapeutic approaches to anti-cancer strategies.

Keywords: 703 retinoblastoma • 624 oncology • 554 immunohistochemistry  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×