April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Accessory Lacrimal Gland (ALG) Gene Expression from Human Muller Muscle Conjunctival Resection (MMCR) Specimens Obtained via Laser Capture Microdissection (LCM)
Author Affiliations & Notes
  • Vinay Aakalu
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Sarmad Hassan Jassim
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Amy Lin
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Assraa H Jaboori
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Pete Setabutr
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Sandeep Jain
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL
  • Footnotes
    Commercial Relationships Vinay Aakalu, None; Sarmad Jassim, None; Amy Lin, None; Assraa Jaboori, None; Pete Setabutr, None; Sandeep Jain, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3115. doi:
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      Vinay Aakalu, Sarmad Hassan Jassim, Amy Lin, Assraa H Jaboori, Pete Setabutr, Sandeep Jain; Accessory Lacrimal Gland (ALG) Gene Expression from Human Muller Muscle Conjunctival Resection (MMCR) Specimens Obtained via Laser Capture Microdissection (LCM). Invest. Ophthalmol. Vis. Sci. 2014;55(13):3115.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

MMCR specimens taken during routine ptosis surgery frequently contain ALG in addition to other tissue types. ALG gene expression patterns have been studied only very recently and the data available is minimal. To determine gene expression patterns of ALG isolated using LCM, including precursor cell genes, using PCR from MMCR specimens that contain multiple tissue types.

 
Methods
 

A Leica LCM was performed to isolate ALG from MMCR specimens. Following RNA extraction, the gene expression patterns of ALG, including precursor genes (ABCG2, CD133, CD49f, and CD90) was determined using PCR.

 
Results
 

We determined a unique pattern of gene expression in ALG, which was compared with known expression patterns of other exocrine glandular tissue. LCM proved an efficient means of isolating ALG from MMCR specimens, which contain multiple cell and tissue types.

 
Conclusions
 

ALG cells can be isolated from MMCR specimens using LCM and a unique pattern of gene expression, including that of precursor genes is presented.

 
 
Accessory Lacrimal Glands before Laser Capture Microdissection
 
Accessory Lacrimal Glands before Laser Capture Microdissection
 
 
After Laser Capture Microdissection of Accessory Lacrimal Glands
 
After Laser Capture Microdissection of Accessory Lacrimal Glands
 
Keywords: 526 eyelid • 576 lacrimal gland • 486 cornea: tears/tear film/dry eye  
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