April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
A NOVEL MURINE MODEL FOR KERATOPROSTHESIS RESEARCH
Author Affiliations & Notes
  • Alja Crnej
    Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, MA
  • Masahiro Omoto
    Schepens Eye Research Institute, Boston, MA
  • Thomas H Dohlman
    Schepens Eye Research Institute, Boston, MA
  • Claes H Dohlman
    Schepens Eye Research Institute, Boston, MA
  • Reza Dana
    Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, MA
    Schepens Eye Research Institute, Boston, MA
  • Footnotes
    Commercial Relationships Alja Crnej, None; Masahiro Omoto, None; Thomas Dohlman, None; Claes Dohlman, None; Reza Dana, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3147. doi:
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      Alja Crnej, Masahiro Omoto, Thomas H Dohlman, Claes H Dohlman, Reza Dana, ; A NOVEL MURINE MODEL FOR KERATOPROSTHESIS RESEARCH. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3147.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To establish a murine model for keratoprosthesis

Methods: A miniature keratoprosthesis (m-KPro) device was created consisting of a poly[methyl methacrylate] front part and a titanium back plate, designed after the Boston KPro, which is in widespread clinical use. BALB/c mice were used and a 2 mm in diameter donor cornea was punched out. After 2 mm trepanation of the syngeneic recipient cornea, extra-capsular crystalline lens extraction was performed. The m-KPro was assembled onto the cornea button in a similar manner to human KPro implantation. The cornea - device complex was secured to the recipient bed with eight interrupted 11-0 sutures. All mice (n=10) were followed for 8 weeks postoperatively.

Results: All m-KPro were successfully implanted and retained in all 10 animals. There were no critical complications such as endophthalmitis, corneal melting, device extrusions, leakage, extensive inflammation, or weight loss in the animals. There was mild to moderate donor and host corneal neovascularization in all cases throughout the follow-up period.

Conclusions: We have established a novel murine model of KPro implantation which mirrors clinical KPro with high fidelity. This model will allow for future studies of immuno-pathological responses to KPro implantation.

Keywords: 575 keratoprostheses  
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