April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Confocal Brillouin scanning microscope for estimation of corneal stiffness
Author Affiliations & Notes
  • Amit Paranjape
    R&D, Avedro Inc., Waltham, MA
  • Sarah Peterson
    R&D, Avedro Inc., Waltham, MA
  • Marc D Friedman
    R&D, Avedro Inc., Waltham, MA
  • David Muller
    R&D, Avedro Inc., Waltham, MA
  • Footnotes
    Commercial Relationships Amit Paranjape, Avedro Inc. (E); Sarah Peterson, Avedro Inc. (E); Marc Friedman, Avedro Inc. (E); David Muller, Avedro Inc. (I)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3721. doi:
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    • Get Citation

      Amit Paranjape, Sarah Peterson, Marc D Friedman, David Muller; Confocal Brillouin scanning microscope for estimation of corneal stiffness. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3721.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To develop a new non-contact confocal Brillouin Scanning Microscope for measurement of corneal stiffness.

Methods: A bench-type Brillouin confocal microscope was developed using a confocal scanning system. Fresh whole porcine globes were obtained <24 hours postmortem in saline on ice from Sioux-preme (Sioux City, IA). 8 Eyes were brought to 37°C and the epithelium debrided using a dull blade. Eyes were then soaked in a solution consisting of 0.1%RF and 0.85% saline for 20 minutes. Brillouin scattering microscopy measurements were performed on the 8 eyes. The corneas were divided into two groups and irradiated with doses of 5.4J/cm2 and 10.8J/cm2 with UVA light at 365nm with an irradiance of 30mW/cm2 pulse 1 sec on 1 second off. Brillouin microscopy was performed again and corneal stiffness values were calculated from the Brillouin shifts for pre and post cross-linking.

Results: The new system was able to perform automated scanning. Corneal stiffness values pre and post crosslinking showed higher stiffness values after cross linking with increased stiffening with dose.

Conclusions: A new confocal Brillouin scanning microscopy system has been demonstrated. This system provides a measure of corneal stiffness pre and post cross-linking of riboflavin/UVA illuminated porcine eyes. Brillouin scanning microscopy may potentially serve as a useful non-contact tool for quantifying the biomechanical changes after cross linking in the cornea.

Keywords: 480 cornea: basic science  
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