April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
3-hydroxykynurenin concentration changes in retina after partial optic nerve crush and NMDA-induced RGC damage in rat
Author Affiliations & Notes
  • Tomasz Choragiewicz
    Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland
    Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany
  • Sebastian Thaler
    Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany
  • Frank Schuettauf
    Centre for Ophthalmology, University of Tuebingen, Tuebingen, Germany
  • Kamila Wertejuk
    Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland
  • Dominika Nowakowska
    Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland
  • Michael J Koss
    University Eye Clinic, J W Goethe-Universitaet FFM, Frankfurt/Main, Germany
  • Tomasz Kocki
    Deptartment of Clinical Pharmacology and Toxicology, Medical University of Lublin, Lublin, Poland
  • Anselm G M Junemann
    Department of Ophthalmology, University of Erlangen-Nuernberg, Erlangen, Germany
  • Waldemar A Turski
    Deptartment of Clinical Pharmacology and Toxicology, Medical University of Lublin, Lublin, Poland
  • Robert Rejdak
    Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland
    Department of Experimental Pharmacology, PAS Medical Research Centre, Warsaw, Poland
  • Footnotes
    Commercial Relationships Tomasz Choragiewicz, None; Sebastian Thaler, None; Frank Schuettauf, None; Kamila Wertejuk, None; Dominika Nowakowska, None; Michael Koss, None; Tomasz Kocki, None; Anselm Junemann, None; Waldemar Turski, None; Robert Rejdak, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 388. doi:
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      Tomasz Choragiewicz, Sebastian Thaler, Frank Schuettauf, Kamila Wertejuk, Dominika Nowakowska, Michael J Koss, Tomasz Kocki, Anselm G M Junemann, Waldemar A Turski, Robert Rejdak; 3-hydroxykynurenin concentration changes in retina after partial optic nerve crush and NMDA-induced RGC damage in rat. Invest. Ophthalmol. Vis. Sci. 2014;55(13):388.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate concentration of 3-hydroxykynurenin (3OH-K) in retinas in a rat model of partial optic nerve crush (PONC) and NMDA-induced damage of rat retinal ganglion cells (RGCs)

Methods: Female Brown Norway rats (Charles River, Wilmington, MA), body weight 140-170g were housed under a 12-hour light-dark cycle. The animals were treated in accordance with the ARVO Statement for the Use of Animals in Vision Research. The rats were anesthetized with an intraperitoneal injection of chloral hydrate (7%, 6ml/kg body weight), local anaesthesia in form of eye drops were also applied. One group of animals received intravitreal NMDA (2µl, 10mM). Control eye received PBS (2µl). In the other group of animals PONC or sham procedure in control eye were performed. On day 2 and 7 eyes were enucleated, retinas dissected and immediately deep frozen. 3OH-K concentration was measured with HPLC.

Results: Retinal concentration of 3OH-K after 2 days was significantly higher in retinas 2 days after both NMDA injection and PONC (27.4% and 40% increases respectively, P<0.05, U Mann-Whitney). After 7 days 3OH-K concentration was significantly higher in retinas after NMDA injection (217% increase, P<0.01) but not after PONC (21% increase, P>0.05).

Conclusions: Retinal concentration of 3OH-K increases in response both to PONC and NMDA-induced RGC damage in rat, suggesting its involvement in RGC degenerative processes.

Keywords: 690 retina: neurochemistry • 615 neuroprotection  
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