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Sun Woong Kim, Taiyoun Rhim; Proteomic analysis in pterygium. Invest. Ophthalmol. Vis. Sci. 2014;55(13):394.
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To identify differentially expressed proteins in pterygium compared to healthy conjunctiva using a proteomic analysis.
Pterygial and healthy conjunctival tissues were obtained from 24 patients undergoing pterygium excision. Total proteins of pterygia and healthy conjunctiva were analyzed by one-dimensional electrophoresis, and protein bands of interest were excised and subjected to LC-MS/MS analysis using Thermo’s Finnigan ProteomeX workstation LTQ linear IT MS (Thermo Electron, San Jose, CA) equipped with an NSI source (San Jose, CA). Using bioinformatics, differentially expressed proteins were classified, and three proteins closely involved in the response to oxidative stress were selected for further validation. Differential expression of these proteins was confirmed by Western blot and immunohistochemistry.
A web-based gene ontology program, DAVID, was used to classify 230 proteins that were differentially expressed in pterygial tissues. Among them, we chose three proteins, aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1), protein disulfide-isomerase A3 (PDIA3) and peroxiredoxin-2 (PRDX2), that were significantly overexpressed in pterygium and further overexpressed in recurrent pterygium. Immunohistochemistry and Western blot analysis confirmed that these three proteins were mainly detected in the basal epithelial layer, and their expression was significantly increased in pterygium compared to normal conjunctiva.
This study reported increased expressions of ALDH3A1, PDIA3, and PRDX2 in pterygia using a proteomic approach. These proteins are presumed to have a protective role against oxidative stress-induced apoptosis. This result is consistent with the hypothesis that oxidative stress is a significant factor in the pathogenesis of pterygium.
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