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Hector Javier Perez-Cano, Ezequiel G Díaz-Benítez, Miriam G Rojas-Tejeda, Molecular Microbilogy; Prevalence of HPV in conjunctiva of healthy subjects in Mexican population. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4449.
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© ARVO (1962-2015); The Authors (2016-present)
Human papillomavirus (HPV) and their genotypes are widely distributed in the world. It has no seasonal incidence, it is one of the sexually transmitted infections more frequent and 16,18,31,33,35,39, 45, 51,52,56,58,59 and 66 genotypes, have been implicated in the development of cervical cancer. HPV has been linked in ocular diseases such as the development of pterygium and ocular surface squamous neoplasia. In this study we determine the presence of HPV in conjunctiva of healthy subjects.
We studied a group of 50 healthy subjects of both sexes over 18 years of age and without ocular pathology. 50 Samples of scraping conjunctival were studied. We perform DNA extraction. The presence of HPV by the technique of the polymerase chain reaction was determined using universal oligonucleotides MY09/MY11. The positive samples were genotyped by terminators fluorescent nucleotide sequencing.
We obtained. 50 samples (27 men and 23 women) aged between 20 and 51 years. The samples analyzed by PCR for the detection of HPV, 2 were positive; it corresponds to 2% of the population. The results grouped by gender corresponding to 3% of the male population and 4% of the female population
The prevalence of HPV found is similar to those reported in other countries. This study highlights the importance of focused preventive measurements to diseases caused by this virus, without forgetting the eye diseases.
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