April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Localization of scleral stem/progenitor cells in murine sclera
Author Affiliations & Notes
  • Pei-Chang Wu
    Ophthalmology, Chang Gung Memorial Hospital - Kaohsiung, Kaohsiung, Taiwan
  • Chia-Ling Tsai
    Dentistry, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan
  • Footnotes
    Commercial Relationships Pei-Chang Wu, None; Chia-Ling Tsai, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4452. doi:
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      Pei-Chang Wu, Chia-Ling Tsai; Localization of scleral stem/progenitor cells in murine sclera. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4452.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: In 2011 we reported the discovery of a group of multipotent mesenchymal stem cells at the sclera, which we named the scleral stem/progenitor cells (SSPCs). The purpose of this study was further to explore the localization of SSPCs in the murine sclera.

Methods: We performed BrdU pulse-chase experiments in C57BL6/J mice. The mice were administered daily intraperitoneal injections of a nucleotide dye, bromodeoxyuridine (Brdu), over 7 consecutive days. The BrdU labeled cells were detected by the immunochemical study in the sclera. As the ABCG and Sca-1 are good markers for stem cells. We also performed the immunofluorescence study to localize the SSPCs.

Results: There were significant more BrdU-positive cells in the anterior sclera in comparison to the posterior sclera. The ABCG and Sca-1 positive cells were also present in the anterior sclera mostly.

Conclusions: The findings are consistent with the presence of a small population of scleral stem/progenitor cells localized along the murine sclera. It may help further studies of scleral diseases such as scleritis and myopia.

Keywords: 721 stem cells • 419 anatomy  
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