April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Controlled Transscleral Dual-drug Delivery by a Polymeric Device Reduces Light-induced Retinal Damage
Author Affiliations & Notes
  • Nobuhiro Nagai
    Graduate School of Medicine, Tohoku University, Sendai, Japan
  • Hirokazu Kaji
    Graduate School of Engineering, Tohoku University, Sendai, Japan
  • Zhaleh Kashkouli Nezhad
    Graduate School of Medicine, Tohoku University, Sendai, Japan
  • Kaori Sampei
    Graduate School of Medicine, Tohoku University, Sendai, Japan
  • Satoru Iwata
    Graduate School of Medicine, Tohoku University, Sendai, Japan
  • Matsuhiko Nishizawa
    Graduate School of Engineering, Tohoku University, Sendai, Japan
  • Yukihiko Mashima
    R-Tech Ueno, Tokyo, Japan
  • Toshiaki Abe
    Graduate School of Medicine, Tohoku University, Sendai, Japan
  • Footnotes
    Commercial Relationships Nobuhiro Nagai, None; Hirokazu Kaji, None; Zhaleh Kashkouli Nezhad, None; Kaori Sampei, None; Satoru Iwata, None; Matsuhiko Nishizawa, None; Yukihiko Mashima, R-Tech Ueno (E); Toshiaki Abe, R-Tech Ueno (F)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 446. doi:
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      Nobuhiro Nagai, Hirokazu Kaji, Zhaleh Kashkouli Nezhad, Kaori Sampei, Satoru Iwata, Matsuhiko Nishizawa, Yukihiko Mashima, Toshiaki Abe; Controlled Transscleral Dual-drug Delivery by a Polymeric Device Reduces Light-induced Retinal Damage. Invest. Ophthalmol. Vis. Sci. 2014;55(13):446.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate the protective effects of a transscleral dual-drug delivery device that can release unoprostone isopropyl (UNO) and edaravone (EDV) at independently controlled release rates against light-induced retinal damage in rats.

Methods: The device consists of a reservoir, controlled-release cover, and drug formulations, which were made of photopolymeized poly(ethyleneglycol) dimethacrylate that partially contains tri(ethyleneglycol) dimethacrylate. These parts were fabricated via a microfabrication technique that used an AutoCAD design. UNO, a prostanoid for antiglaucoma eyedrops marketed in Japan, and EDV, a potent free radical scavenger, were loaded in the device. High-performance liquid chromatography was used to evaluate the release amount of UNO and EDV. After the devices were placed onto the sclera of eyes in rats for 1 week, the rats were exposed to 8000 lux of white fluorescent light for 24 h, then flash electroretinograms were recorded. Histological examinations were perfomred to evaluate the thickness of the outer nuclear layer and the number of apoptotic cells.

Results: UNO and EDV were simultaneously released with zero-ordered kinetics from the device. Trannsscleral co-administration of EDV and UNO by the device reduced loss of retinal function assessed by electroretinograms compared to single administration. The device also precluded the reductions of retinal thickness and the number of apoptotic cells after light exposure.

Conclusions: Co-delivery of EDV and UNO by our device attenuates light-induced retinal damage morphologically and functionally. Controlled dual-drug delivery using our device can protect retinal function from light damage by a less invasive transscleral administration.

Keywords: 688 retina • 503 drug toxicity/drug effects • 615 neuroprotection  
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