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Genea Edwards, Katyayini Aribindi, Yenifer S Guerra, Richard K Lee, Sanjoy K Bhattacharya; Phospholipid Profiles of Control and Glaucomatous Human Aqueous Humor. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4525.
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To compare phospholipid (phosphatidylcholine, phosphatidylserine, phosphoethanolamine and phosphatidylinositol) profiles of human control and glaucomatous aqueous humor (AH).
AH was procured during routine cataract surgery from primary open angle (POAG) and normal patients (n=20 each) adhering to the Tenets of the Declaration of Helsinki. Lipid extraction was performed using modifications of the Bligh and Dyer method, protein concentration was estimated using the Bradford colorimetric assay, and for select protein concentration was confirmed with PHAST gel densitometry. All four classes of phospholipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards.
Comparative profiles of phosphatidylcholines, phosphatidylserines, phosphoethanolamines and phosphatidylinositols between control and glaucomatous AH showed several common species. A number of unique lipids in all four phospholipid classes were also identified in control AH that were absent in glaucomatous AH and vice versa.
A number of phospholipid species were found to be uniquely present in control, but absent in glaucomatous AH and vice versa.
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