April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Phospholipid Profiles of Control and Glaucomatous Human Aqueous Humor
Author Affiliations & Notes
  • Genea Edwards
    Ophthalmology, Bascom Palmer Eye Institute, Miami, FL
    Biochemistry & Molecular Biology, University of Miami Miller Medical School, Miami, FL
  • Katyayini Aribindi
    Ophthalmology, Bascom Palmer Eye Institute, Miami, FL
  • Yenifer S Guerra
    Ophthalmology, Bascom Palmer Eye Institute, Miami, FL
  • Richard K Lee
    Ophthalmology, Bascom Palmer Eye Institute, Miami, FL
  • Sanjoy K Bhattacharya
    Ophthalmology, Bascom Palmer Eye Institute, Miami, FL
    Biochemistry & Molecular Biology, University of Miami Miller Medical School, Miami, FL
  • Footnotes
    Commercial Relationships Genea Edwards, None; Katyayini Aribindi, None; Yenifer Guerra, None; Richard Lee, None; Sanjoy Bhattacharya, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4525. doi:
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      Genea Edwards, Katyayini Aribindi, Yenifer S Guerra, Richard K Lee, Sanjoy K Bhattacharya; Phospholipid Profiles of Control and Glaucomatous Human Aqueous Humor. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4525.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To compare phospholipid (phosphatidylcholine, phosphatidylserine, phosphoethanolamine and phosphatidylinositol) profiles of human control and glaucomatous aqueous humor (AH).

Methods: AH was procured during routine cataract surgery from primary open angle (POAG) and normal patients (n=20 each) adhering to the Tenets of the Declaration of Helsinki. Lipid extraction was performed using modifications of the Bligh and Dyer method, protein concentration was estimated using the Bradford colorimetric assay, and for select protein concentration was confirmed with PHAST gel densitometry. All four classes of phospholipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards.

Results: Comparative profiles of phosphatidylcholines, phosphatidylserines, phosphoethanolamines and phosphatidylinositols between control and glaucomatous AH showed several common species. A number of unique lipids in all four phospholipid classes were also identified in control AH that were absent in glaucomatous AH and vice versa.

Conclusions: A number of phospholipid species were found to be uniquely present in control, but absent in glaucomatous AH and vice versa.

Keywords: 583 lipids • 427 aqueous • 568 intraocular pressure  
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