April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effects of Topical Adiponectin on Epithelial Wound Healing after Corneal Alkali Burn
Author Affiliations & Notes
  • Zhengri Li
    Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Mi Sun Sung
    Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Hyun Ho Jung
    Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Soo Hyun Park
    Bio-therapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea
  • Je Moon Woo
    Department of Ophthalmology, Ulsan University Hospital, Ulsan, Republic of Korea
  • Kyung Chul Yoon
    Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Footnotes
    Commercial Relationships Zhengri Li, None; Mi Sun Sung, None; Hyun Ho Jung, None; Soo Hyun Park, None; Je Moon Woo, None; Kyung Chul Yoon, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4681. doi:
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      Zhengri Li, Mi Sun Sung, Hyun Ho Jung, Soo Hyun Park, Je Moon Woo, Kyung Chul Yoon; Effects of Topical Adiponectin on Epithelial Wound Healing after Corneal Alkali Burn. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4681.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigated the wound healing effect of adiponectin eye drops following alkali burn in the human corneal epithelium.

Methods: Adenovirus 12-SV40 hybrid transformed human corneal epithelial cells (HCE-2) were used. Chemical burn was induced by 0.01N NaOH in HCE-2. After chemical injury, HCE-2 was treated with 0.0001%, 0.001% and 0.01% adiponectin, or 0.1% hyaluronic acid (HA). Cell viability was measured by the MTT assay. The wound healing rate of injured HCE-2 was also evaluated with migration assay at 4, 8, 12 and 24 hours.

Results: Cellular viability was not changed by adiponectin with various concentrations. HCE-2 treated with 0.001% and 0.01% adiponectin showed a significant improvement in wound healing rate from 4 hours compared with the HA-treated groups (P<0.05). However, there were no significant differences between the 0.0001% adiponectin and HA-treated groups.

Conclusions: Application of adiponectin (0.001-0.01%) did not change the cellular viability in HCE-2 and significantly increased the ability of epithelial migration after alkali injury, suggesting that adiponectin treatment can promote the epithelial wound healing in the cornea.

Keywords: 765 wound healing • 482 cornea: epithelium • 449 cell survival  
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