Purchase this article with an account.
Zhengri Li, Mi Sun Sung, Hyun Ho Jung, Soo Hyun Park, Je Moon Woo, Kyung Chul Yoon; Effects of Topical Adiponectin on Epithelial Wound Healing after Corneal Alkali Burn. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4681.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigated the wound healing effect of adiponectin eye drops following alkali burn in the human corneal epithelium.
Adenovirus 12-SV40 hybrid transformed human corneal epithelial cells (HCE-2) were used. Chemical burn was induced by 0.01N NaOH in HCE-2. After chemical injury, HCE-2 was treated with 0.0001%, 0.001% and 0.01% adiponectin, or 0.1% hyaluronic acid (HA). Cell viability was measured by the MTT assay. The wound healing rate of injured HCE-2 was also evaluated with migration assay at 4, 8, 12 and 24 hours.
Cellular viability was not changed by adiponectin with various concentrations. HCE-2 treated with 0.001% and 0.01% adiponectin showed a significant improvement in wound healing rate from 4 hours compared with the HA-treated groups (P<0.05). However, there were no significant differences between the 0.0001% adiponectin and HA-treated groups.
Application of adiponectin (0.001-0.01%) did not change the cellular viability in HCE-2 and significantly increased the ability of epithelial migration after alkali injury, suggesting that adiponectin treatment can promote the epithelial wound healing in the cornea.
This PDF is available to Subscribers Only