April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
EFFECT OF DIFFERENT FORTIFIED ANTIBIOTIC EYE DROPS ON HUMAN AND BOVINE CORNEAL CELLS “IN VITRO”
Author Affiliations & Notes
  • Anxo Fernandez Ferreiro
    Pharmacy, Xerencia de Xestión Integrada de Santiago de Compostela (SERGAS), Santiago de Compostela, Spain
    Pharmacy and Pharmaceutical Technology, University of Santiago de Compostela USC, Santiago de Compostela, Spain
  • Maria Santiago-Varela
    Ophthalmology, Xerencia de Xestión Integrada de Santiago de Compostela (SERGAS), Santiago de Compostela, Spain
  • Maria Pardo
    Instituto de Investigación Sanitaria (IDIS), Servicio Galego de Saúde (SERGAS), Santiago de Compostela, Spain
  • Miguel Gonzalez Barcia
    Pharmacy, Xerencia de Xestión Integrada de Santiago de Compostela (SERGAS), Santiago de Compostela, Spain
  • Antonio Pineiro-Ces
    Ophthalmology, Xerencia de Xestión Integrada de Santiago de Compostela (SERGAS), Santiago de Compostela, Spain
  • Jose Blanco Mendez
    Pharmacy and Pharmaceutical Technology, University of Santiago de Compostela USC, Santiago de Compostela, Spain
  • Maria Jesus Lamas Díaz
    Pharmacy, Xerencia de Xestión Integrada de Santiago de Compostela (SERGAS), Santiago de Compostela, Spain
  • Francisco Otero Espinar
    Pharmacy and Pharmaceutical Technology, University of Santiago de Compostela USC, Santiago de Compostela, Spain
  • Footnotes
    Commercial Relationships Anxo Fernandez Ferreiro, None; Maria Santiago-Varela, None; Maria Pardo, None; Miguel Gonzalez Barcia, None; Antonio Pineiro-Ces, None; Jose Blanco Mendez, None; Maria Jesus Lamas Díaz, None; Francisco Otero Espinar, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4891. doi:
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      Anxo Fernandez Ferreiro, Maria Santiago-Varela, Maria Pardo, Miguel Gonzalez Barcia, Antonio Pineiro-Ces, Jose Blanco Mendez, Maria Jesus Lamas Díaz, Francisco Otero Espinar; EFFECT OF DIFFERENT FORTIFIED ANTIBIOTIC EYE DROPS ON HUMAN AND BOVINE CORNEAL CELLS “IN VITRO”. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4891.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The aim of this study was to compare the cytotoxic effect of some of the most widely used fortified antibiotic eye drops on different primary corneal cell lines using real-time monitoring of dynamic changes induced by cell-toxicant interaction

Methods: Six fortified antibiotic eye drops (Cefazolin 50 mg/ml, Vancomycin 50 mg/ml, Amikacin 33 mg/ml, Gentamicin 15 mg/ml, Imipenem 5 mg/ml, Ceftazidime 50 mg/ml) were selected to be tested in four different primary corneal cell cultures: Human Corneal Keratocytes (HCK) and Human Corneal Epithelial (HCE), Bovine Corneal Keratocyte (BCK) and Bovine Corneal Epithelial (BCE). Cell cytotoxicity was assessed by using the label-free and real-time monitoring xCELLigence system (ACEA Biosciences, San Diego, CA). Under this platform, Cell index (CI) was the parameter used to represent cell status based on the measured electrical impedance. Briefly, 3000 cells/well (E-plates 16 wells) were seeded and incubated for 24 hours until the CI reached a range of 1.0- 1.2 indicating about 60% cell confluence. At that point, cell culture medium was aspirated to perform cell treatment with the fortified eye drops at 1:10 dilution in culture medium. Assays were performed in triplicates for each cell line and for each antibiotic

Results: Kinetic curve surviving rates show that all fortified eye drops studied, except vancomycin and imipenem, induced a gradually decline in the cell surviving rate over a 24 hours exposure period, for all the studied cell lines. This effect suggests that amikacin, gentamicin, cefazolin and ceftazidime cause a significant corneal cellular toxicity. An analysis of cell surviving rate kinetic curves shows that these effects were time and dose dependent. More studies are actually in course to estimate the best non-toxic concentration, the cell recovery rate and the necessary time for this recovery. On the other hand, the kinetic curves for vancomicyn and imipenem surviving rate show a fast initial increase in the CI value that deserves further studies

Conclusions: These results can be particularly relevant to estimate the optimal antibiotic concentration to be applied in clinical situations to avoid a toxic effect

Keywords: 422 antibiotics/antifungals/antiparasitics • 449 cell survival • 480 cornea: basic science  
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