April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effects of Nanoparticle Exposure on the Growth of Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • Bedia Karakocak
    Chemical Engineering, Washington University, St. Louis, MO
    Ophthalmology, Washington University School of Medicine, St. Louis, MO
  • Ramesh Raliya
    Chemical Engineering, Washington University, St. Louis, MO
  • Joshua Davis
    Ophthalmology, Washington University School of Medicine, St. Louis, MO
    Research, VA Health Care System, St. Louis, MO
  • Paul David Hamilton
    Ophthalmology, Washington University School of Medicine, St. Louis, MO
    Research, VA Health Care System, St. Louis, MO
  • Pratim Biswas
    Chemical Engineering, Washington University, St. Louis, MO
  • Nathan Ravi
    Chemical Engineering, Washington University, St. Louis, MO
    Ophthalmology, Washington University School of Medicine, St. Louis, MO
  • Footnotes
    Commercial Relationships Bedia Karakocak, None; Ramesh Raliya, None; Joshua Davis, None; Paul Hamilton, None; Pratim Biswas, None; Nathan Ravi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4899. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Bedia Karakocak, Ramesh Raliya, Joshua Davis, Paul David Hamilton, Pratim Biswas, Nathan Ravi, ; Effects of Nanoparticle Exposure on the Growth of Retinal Pigment Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4899.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

Various kind of intentionally created metal and metal oxide nanoparticles have been widely used in medicine, engineering, electronics, agriculture and allied sciences. However, their toxicity to the ocular tissue is still not clear. In the present study we used three different kinds of nanoparticles i.e. gold, titanium dioxide (TiO2), and organosilica. Their toxic effects in retinal pigment epithelial cell line ARPE 19 were determined in vitro.

 
Methods
 

Nanoparticles of Au, TiO2 and organosilica were synthesized according to Wu et al., 2010, Katoch et al., 2012, and Irmukhametova et al. 2011 respectively. NPs were characterized by UV-VIS, Size distribution and Zeta potential by DLS, and morphological analysis by TEM (Table 1). To evaluate biocompatibility on retinal pigmented epithelial cells (ARPE-19) we used thiazolyl blue (MTT) assay. A standard growth curve was established for ARPE-19 cells and mathematical models based on the Gompertz function and the Ontogenetic General Model set by West et. al., 2001. These values were fed into the mathematical models to solve for the treatment effect and whether it is apoptotic or inhibitory to the cell cycle.

 
Results
 

Both the Gompertz and Ontogenetic General Model curves created show the ability to accurately fit the data (R2 of 0.9773 and 0.9645 respectively) and can reproduce established constants of human tissue growth (Fig. 1). With treatment added into the model we are able to represent and predict, through the variations in concentration, what effects the “pollutants” have on the cell cycle. The MTT assay shows an LC50 for TiO2 particles around 350 ppm while for gold particles the lethal dose is below 1 ppm, and for the organosilica particles the LC50 exceeds 1000 ppm. MTT assay is not compatible with gold nanoparticle exposure at the concentrations we have run, due to nanoparticle absorbance of yellow light.

 
Conclusions
 

Nanoparticles were successfully synthesized on the basis of previously established protocols. Of the three NPs evaluated on ARPE19 cells, the order of toxicity was highest in Au followed by TiO2 and lastly organosilica NPs. Root cause of maximum toxicity by Au NPs is may be due to positive surface charge and its oxidation state of +3.

 
 
Table 1: Nanoparticle characterization
 
Table 1: Nanoparticle characterization
 
 
Figure 1: Ontogenetic General Model curve for ARPE 19 cells fit to experimental data. Showing equation and R2 fit.
 
Figure 1: Ontogenetic General Model curve for ARPE 19 cells fit to experimental data. Showing equation and R2 fit.
 
Keywords: 620 ocular irritancy/toxicity testing • 607 nanotechnology  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×