Purchase this article with an account.
Rui Cui, Qingjun Lu, Kun Li, Na Li; Chitosan promoted the corneal epithelial wound healing via activation of ERK MAPK Pathway. Invest. Ophthalmol. Vis. Sci. 2014;55(13):499.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Chitosan has ability to promoting the viability of rabbits corneal epithelial cells(RCECs) are estimated by MTT assay. However, the molecular mechanism by which chitosan functions is unknown. We investigated the possible effects of chitosan on extracellular regulated kinase (ERK) and p38 pathways in rabbit corneal wound healing.
Establishing the corneal epithelium mechanical damaged model, followed by the respectively treatments with normal saline(group A), 0.5%chitosan(group B), 20μg/ml recombinant human epidermal growth factor (group C), Observing the corneal wound area by corneal fluorescein staining at timing series. RCECs were cultured in vitro, epithelial cell proliferation was evaluated with Ki67 antibody. To measure in vitro wound closure, confluent RCECs were wounded by a linear scraping with a sterile plastic tip in the center of the well. Cells were incubated for 24 hours with or without chitosan.Phosporylation of ERK and p38 were analyzed by western blot in the presence or absence of inhibitors. 8 mm epithelial debridement wounds were made in rabbits corneas in organ culture in the presence of chitosan with or without ERK pathway inhibitor.
The corneal unhealing area of Group B was statistically significant smaller than group A (p<0.05) and no significant difference with group C (p>0.05) in 24hour, 48hour and 72hour. Immunostaining with Ki67 and migrating assay showed that chitosan could up-regulate the cell proliferation and promote the cell migration. Chitosan activated ERK in 5min to 30min but not p38 and PD98059 inhibited the chitosan-stimulated ERK phosphorylation. Chitosan increased corneal epithelial wound closure in organ culture and ERK inhibition with PD98059 blocked the effect of chitosan on wound healing.
Chitosan promoted corneal epithelial proliferation and migration during the wound healing in rabbits eyes. Chitosan-stimulated epithelial wound healing is partially mediated through the activation of ERK pathway but not p38 pathway.
This PDF is available to Subscribers Only