April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Corneal Photodynamic Ablation of Lymphatic Vessels improves Corneal Graft Survival
Author Affiliations & Notes
  • Laura Schollhorn
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Franziska Bucher
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Felix Bock
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Claus Cursiefen
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Footnotes
    Commercial Relationships Laura Schollhorn, None; Franziska Bucher, None; Felix Bock, None; Claus Cursiefen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5048. doi:
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    • Get Citation

      Laura Schollhorn, Franziska Bucher, Felix Bock, Claus Cursiefen, ; Corneal Photodynamic Ablation of Lymphatic Vessels improves Corneal Graft Survival. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5048.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Corneal (lymph)angiogenesis is a prime risk-factors for immune rejection after transplantation. We recently presented a technique to regress pre-existing pathological corneal lymphatic vessels prior to transplantation by photodynamic therapy (PDT). Here we analysed the effect of photodynamic regression of corneal lymphatic vessels on subsequent corneal graft survival.

Methods: The murine model of high-risk keratoplasty was used by placing three interrupted 11-0 sutures into the corneal stroma of BALB/c mice and leaving them in place for 2 weeks. Thereafter, the treatment group received an intrastromal injection of verteporfin (controls: PBS) followed by PDT. Subsequently, one, two or three weeks later penetrating keratoplasty was performed with C57BL/6 donors (all mice female). Postoperative survival of the grafts was analyzed over 8 weeks subsequently.

Results: Performing PK in high risk eyes two or three weeks after corneal stromal PDT resulted in significantly higher graft survival rates (p<0.005 and p<0.05, respectively; n=8). When performing the corneal transplantation one week after corneal stromal PDT, no significant difference in graft survival could be detected (p>0.05; n=7).

Conclusions: This study for the first time shows that PDT after corneal intrastromal verteporfin injection is a new “preconditioning strategy” to reduce pre-existing corneal lymphatic vessels prior to transplantation and thereby to prevent allograft rejection in the high-risk situation.

Keywords: 555 immunomodulation/immunoregulation • 741 transplantation • 479 cornea: clinical science  
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