April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effect of carboxymethyl chitosan on the corneal alkali burn injury in dogs
Author Affiliations & Notes
  • Young Sam Kwon
    Veterinary Surgery, College of Vet Med, Kyungpook National University, Daegu, Republic of Korea
  • Sae Kwang Ku
    College of Oriental Medicine, Daegu Haany University, Daegu, Republic of Korea
  • Dong Gook Lee
    Jukjeon Animal Medical Center, Daegu, Republic of Korea
  • Kwang Ho Jang
    Veterinary Surgery, College of Vet Med, Kyungpook National University, Daegu, Republic of Korea
  • Footnotes
    Commercial Relationships Young Sam Kwon, None; Sae Kwang Ku, None; Dong Gook Lee, None; Kwang Ho Jang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5165. doi:
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      Young Sam Kwon, Sae Kwang Ku, Dong Gook Lee, Kwang Ho Jang; Effect of carboxymethyl chitosan on the corneal alkali burn injury in dogs. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5165.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: This study was performed to evaluate the effect of topical application of carboxymethyl chitosan (CMC) on corneal alkali burn injury in dogs.

Methods: Fifteen healthy adult male Beagle dogs were used and the corneal alkalie burn was induced by applying a filter paper soaked in NaOH on the cornea for 60 seconds Normal saline (control group), 0.3% ofloxacin, and 0.5% CMC eye drops were applied for 10 days after alkali injury. Corneal epithelial defect and neovascularization were evaluated on days 1, 3, 7, and 10. Hematoxylin-eosin, masson’s trichrome and immunohistochemical stainings were performed to evaluate general histopathology and the expression patterns of apoptotic markers (caspase-3 and PARP), proinflammatory cytokines (TNF-α, IL-1β), inflammatory mediators (COX-2, iNOS) and angiogenesis markers (VEGF).

Results: Corneal defect and neovascularization scores were significantly decreased in CMC treated group compared with control group (p<0.05). Cornea and conjunctiva epithelial thicknesses significantly increased in ofloxacin group and CMC group (p<0.05). Histologically, the thickness of damaged corneal and the number of inflammatory cells significantly reduced in ofloxacin and CMC treated cornea (p<0.05). In addition, the expressions of caspase-3, PARP, TNF-α, IL-1β, COX-2, iNOS, and VEGF were significantly inhibited in the ofloxacin and CMC groups. Particularly, CMC significantly decreased the corneal damage and the expressions of the markers as above compared with ofloxacin.

Conclusions: This study suggests that topical application of 0.5% CMC may have a beneficial effect on corneal alkali burn injury in dogs by regeneration of damaged cornea and alleviation of its inflammatory and apoptotic processes.

Keywords: 482 cornea: epithelium • 765 wound healing  
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