April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Adipose Derived Stem Cells (ADS) for Ocular Surface Regeneration.
Author Affiliations & Notes
  • Ricardo Pedro Casaroli-Marano
    Department of Surgery, University of Barcelona, Barcelona, Spain
    Transplant Services Foundation, Hospital Clínic de Barcelona, Barcelona, Spain
  • Eva M Martínez-Conesa
    Transplant Services Foundation, Hospital Clínic de Barcelona, Barcelona, Spain
  • Nuria Nieto-Nicolau
    Department of Cell Biology, University of Barcelona, Barcelona, Spain
  • Francisco Arnalich-Montiel
    Cell Engineering Lab, Hospital de La Paz (IdiPAZ), Madrid, Spain
  • Sherezade Fuentes-Julian
    Cell Engineering Lab, Hospital de La Paz (IdiPAZ), Madrid, Spain
  • Maria P De Miguel
    Cell Engineering Lab, Hospital de La Paz (IdiPAZ), Madrid, Spain
  • Footnotes
    Commercial Relationships Ricardo Casaroli-Marano, None; Eva Martínez-Conesa, None; Nuria Nieto-Nicolau, None; Francisco Arnalich-Montiel, None; Sherezade Fuentes-Julian, None; Maria De Miguel, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5183. doi:
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      Ricardo Pedro Casaroli-Marano, Eva M Martínez-Conesa, Nuria Nieto-Nicolau, Francisco Arnalich-Montiel, Sherezade Fuentes-Julian, Maria P De Miguel; Adipose Derived Stem Cells (ADS) for Ocular Surface Regeneration.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5183.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The use of stem cells is promising for future cell-based therapies such as tissue regeneration and engineering. Mesenchymal stem cells (MSCs) derived from adult human adipose tissue (ADS) manifest multilineage differentiation capacity. However, their plasticity to differentiate into epithelial characteristics still remains little well-known. The corneal epithelium is maintained by limbal stem cells. Transplantation of autologous corneal stem cells it is not possible in several cases in which bilateral disease produces total corneal stem-cell deficiency. For this reason, the use of autologous ADS as a source of cells for the ocular surface reconstruction is being studied.

Methods: Pool of ADS cells from different healthy donors was generated. The plasticity, cellular and molecular characterization and ADS in vitro behavior to assumes epithelial-like characteristic were studied. Several limbic cell markers (p63, ABCG2, NGFr) and a set of epithelial markers (cytokeratins 3 and 12, integrins, EGFr and E-cadherin) have been analyzed by RT-PCR methods. After five days in cultures ADS cells were seeded onto human amniotic membrane and applied in a rat model of limbic deficiency. Histopathological and molecular analyses were carried-out between 21 and 28 days.

Results: ADS cells can assume epithelial-like phenotypes when cultured into CnT-30 or SHEM media after 5 days in culture. Expression of an epithelial marker set was more evident in SHEM between 3rd and 7th days in culture. Histopathological studies revealed restructuring of corneal surface, more evident with ADS cells cultured in SHEM. PCR experiments with sequencing methods demonstrated the presence of human CK12 in ADS cells and in corneas of murine in vivo model.

Conclusions: ADS cells could be a potential cell type for ocular surface regenerative medicine, and the epithelial regeneration and the expression of CK12 in murine model of limbic deficiency after 3 weeks may support this hypothesis.

Keywords: 687 regeneration • 480 cornea: basic science • 741 transplantation  
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