April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Peripheral immune response in human toxoplasmosis depends on clinical condition but not on the antigen
Author Affiliations & Notes
  • Alejandra de-la-Torre
    Immunology, Universidad del Rosario, Bogotá, Colombia
    Biomedical Sciences/ Parasitology, Universidad del Quindío, Armenia, Colombia
  • Elizabeth Torres- Morales
    Biomedical Sciences/ Parasitology, Universidad del Quindío, Armenia, Colombia
  • Laura Taborda
    Biomedical Sciences/ Parasitology, Universidad del Quindío, Armenia, Colombia
  • Nestor I Cardona
    Biomedical Sciences/ Parasitology, Universidad del Quindío, Armenia, Colombia
  • Juan C sepúlveda-Arias
    Immunology/Infection, Universidad tecnológica de Pereira, Pereira, Colombia
  • Manuel A Patarroyo
    Molecular biology/Immunology, Fundacion Instituto de Inmunologia de Colombia (FIDIC), Bogotá, Colombia
  • Jorge E Gomez-Marin
    Biomedical Sciences/ Parasitology, Universidad del Quindío, Armenia, Colombia
  • Footnotes
    Commercial Relationships Alejandra de-la-Torre, None; Elizabeth Torres- Morales, None; Laura Taborda, None; Nestor Cardona, None; Juan sepúlveda-Arias, None; Manuel Patarroyo, None; Jorge Gomez-Marin, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5280. doi:
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      Alejandra de-la-Torre, Elizabeth Torres- Morales, Laura Taborda, Nestor I Cardona, Juan C sepúlveda-Arias, Manuel A Patarroyo, Jorge E Gomez-Marin, ; Peripheral immune response in human toxoplasmosis depends on clinical condition but not on the antigen. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5280.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: The peripheral immune response is incompletely known in human toxoplasmosis. We aimed to study this response and the cytokine production regarding P30 and ROP 18 protein-derived Toxoplasma peptides.

Methods: The specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) were determined in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, 9 with congenital toxoplasmosis and 8 Toxoplasma negative people. A Beckman-Coulter FC500n flow cytometer was used for determining antigen-specific T-cells (CD3+CD4+ or CD3+CD8+ cells) in peripheral blood culture. IFNg and IL10 levels were determined in culture supernatants.

Results: Specific CD4+ and CD8+ T-cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response towards Th1 in congenitally-infected children and virulent peptide 2017 induced a Th2 response in chronically-infected, asymptomatic people.

Conclusions: An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.

Keywords: 734 toxoplasmosis • 553 immune tolerance/privilege • 451 chorioretinitis  
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