April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Clinical and functional evaluation of experimental autoimmune uveitis in B10.RIII mice: monophasic versus biphasic forms
Author Affiliations & Notes
  • Jun Chen
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
    Laboratory of Immunology, National Eye Institute, Bethesda, MD
  • Haohua Qian
    Visual Function Core, National Eye Institute, Bethesda, MD
  • Chi-Chao Chan
    Laboratory of Immunology, National Eye Institute, Bethesda, MD
  • Rachel R Caspi
    Laboratory of Immunology, National Eye Institute, Bethesda, MD
  • Footnotes
    Commercial Relationships Jun Chen, None; Haohua Qian, None; Chi-Chao Chan, None; Rachel Caspi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5305. doi:
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      Jun Chen, Haohua Qian, Chi-Chao Chan, Rachel R Caspi; Clinical and functional evaluation of experimental autoimmune uveitis in B10.RIII mice: monophasic versus biphasic forms. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5305.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Non-infectious uveitis in humans is an autoimmune disease of the retina and uvea that leads to blindness. Its laboratory equivalent is experimental autoimmune uveitis (EAU) induced in susceptible rodents by immunization with retinal antigens. The murine model of EAU has been particularly useful, permitting to dissect basic mechanisms as well as serving as a template for translational therapies. The B10.RIII mouse strain is the most susceptible strain known. To characterize EAU in the B10.RIII strain, we used multiple methodologies including non-invasive assessments in comparison with histology for disease evaluation.

 
Methods
 

EAU was induced in B10.RIII mice by immunization with IRBP161-180 in the absence of pertussis toxin. Disease was evaluated longitudinally using non-invasive clinical assessments by fundus examination and photography, optical coherence tomography and functional evaluation by electroretinography (ERG), which were then compared to histopathology.

 
Results
 

The EAU model in the B10.RIII strain had been thought until now to only manifest an acute-monophasic pattern of disease. Unlike previously reported, we found that IRBP-immunized EAU in B10.RIII mice exhibited two distinct patterns of disease depending on clinical scores developed after onset: (i) severe monophasic form of EAU with diffuse/extensive destruction of the retina, followed by a rapid retinal atrophy and loss of visual signal two weeks post immunization, or (ii) lower grade of EAU with an initial acute phase, followed by a prolonged chronic phase and partial recovery of the ERG response, culminating in focal retinal degeneration and loss of vision after 6-7 months.

 
Conclusions
 

We demonstrate for the first time that EAU model in the B10.RIII strain can be either monophasic or biphasic, with distinguishing features. These findings can affect the choice of animal model of human uveitis as a platform to evaluate effects of investigational therapeutic modalities.

  
Keywords: 746 uveitis-clinical/animal model • 551 imaging/image analysis: non-clinical • 510 electroretinography: non-clinical  
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