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Rebecca Marian McQuaid, JiaJun Li, Michael Mrochen, Brian Vohnsen, ; Lateral Diffusion Coefficient in CXL with Corneal Channels. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5535.
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Recent studies involving corneal cross-linking (CXL) have investigated other methods of riboflavin delivery to the cornea such as Iontophoresis, femtosecond laser-created pockets and trans-epithelium CXL. Standard CXL sees riboflavin diffusion to the cornea over a 30 minutes interval. In this study, we investigate a new method of riboflavin delivery to the cornea without removal of the corneal epithelium. The purpose is to gain insight into the temporal dynamics of corneal riboflavin diffusion via surgically-created intra-stromal channels.
Porcine globes were obtained from the local slaughterhouse 4 hours post mortem and kept at a temperature of 4 degrees Celsius. An incision was made using a cannula needle, followed by insertion of keraring (Mediphacos Ltd) to create a channel in the cornea for riboflavin diffusion. Monitoring of the cornea with a CCD camera facilitates the study of diffusion characteristics in real time.
The results show that riboflavin can be effectively injected into the cornea via the created diffusion channels without removal of the epithelium layer. The recorded videos show characteristic time-scales on the order of seconds to minutes from which a characteristic diffusion coefficient can be derived on the basis of Fick’s 2nd law of diffusion.
This study investigated the distribution of riboflavin diffusion in the cornea through creation of surgically-created channels in post-mortem porcine eyes. This could be an effective method for riboflavin application for CXL without the need to remove the epithelial layer and thus with reduced patient discomfort..
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