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Kyung Chul Yoon, Zhengri Li, Je Moon Woo, Soo Hyun Kim, Jee Bum Lee; Blue Light-induced Oxidative Stress in Human Corneal Epithelial Cells: Protective Effect of Ethanol Extracts from Mixture of Various Medicinal Plants. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5538.
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To investigate the effects of visible light on human corneal epithelial cells and the impact of natural antioxidants on oxidative stress produced by light overexposure.
Light emitting diode with various wavelengths (410-830nm) was used to irradiate on human corneal epithelial cells, and then cell viability was assessed. Production of reactive oxygen species (ROS) was analyzed using the 2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA). Ethyl alcohol (EtOH) extracts were prepared from the mixture of various medicinal plants. After application of the EtOH extracts, free-radical-scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The induction of antioxidant enzymes including heme oxygenase-1 (HO-1), peroxireoxin-1 (Prx-1), catalase (CAT), and superoxide dismutase-2 (SOD-2) and inhibitory capability of ROS by the extracts were evaluated by real time-PCR and DCF-DA in human corneal epithelial cells.
The viability of human corneal epithelial cells was diminished after irradiation of blue light (above 10J at 410nm and 50J at 480nm). ROS production by blue light irradiation increased in a dose dependant manner. EtOH extracts had potent radical scavenging activity. The application of the extracts increased the expression of HO-1, Prx-1 , CAT, and SOD-2 and attenuated ROS production by blue light.
Overexposure of blue light (410-480nm) may have a harmful impact on human corneal epithelial cells compared to other visual light wavelengths. Medicinal plant extracts can have potent protective effects on blue light-induced oxidative stress.
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