April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Schlemm’s canal (SC) and Distal Aqueous Outflow Pathways: New scanning EM (SEM) Preparation Technique Permits Identifying Unique Structural Relationships.
Author Affiliations & Notes
  • Elizabeth Martin
    Ophthalmology, University of Washington, Seattle, WA
  • Yi Jiang
    Ophthalmology, University of Washington, Seattle, WA
  • Murray A Johnstone
    Ophthalmology, University of Washington, Seattle, WA
  • Footnotes
    Commercial Relationships Elizabeth Martin, None; Yi Jiang, None; Murray Johnstone, Alcon (C), Allergan (P), Cascade Ophthalmics (C), Healionics (C), Ivantis (C), University of Washington C4C (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5683 - A0385. doi:
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      Elizabeth Martin, Yi Jiang, Murray A Johnstone; Schlemm’s canal (SC) and Distal Aqueous Outflow Pathways: New scanning EM (SEM) Preparation Technique Permits Identifying Unique Structural Relationships.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5683 - A0385.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To describe a new SEM protocol that permits exploring complex relationships between SC, collector channel ostia (CCO) and intrascleral collector channels running parallel to SC lumen (ISPC). The nature of the distal outflow system resistance remains uncertain and has been difficult to characterize with SEM because SC is normally little more than a potential space. We hypothesized that SC viscoelastic dilation, followed by frontal sections through the outflow system would provide an improved understanding of regional relationships.

 
Methods
 

Nemestrina monkey eyes (n=4), 31 limbal segments, Healon viscoelastic used to dilate SC, 150 µm cannula, micromanipulator. Frontal sections transected the outflow system. SEM after fixation, critical point drying, sputtering. Image analysis with Fiji. SC length, ISPC number and dimensions, number of CCO communicating with ISPC, CCO orientation relative to SC and ISPC were measured. Thickness of narrow scleral septum (NSS) separating SC and ISPC between CCO, the number of structures spanning directly between SC inner and outer wall (SSSC) as well as SSSC relationships to CCO and the septa between SC and ISPC were characterized.

 
Results
 

Of the 31 segments, 16 (51.6%) segments had ISPC that coursed circumferentially parallel to SC. Mean SC length within segments was 1130 µm (446-2136µm, SD 586µm). Length of ISPC was 565µm (55-1520µm, SD 486µm), representing 50% of the length of SC. In the 16 segments with ISPC, 20 visible CCO were identified. CCO were arranged perpendicular to SC and ISPC. Mean CCO diameter was 67 µm (27-131µm, SD 36µm). NSS separated each of the ISPC from SC with a rather uniform diameter between ostia of 23µm, SD11µm. Eight segments had SSSC spanning between SC inner wall endothelium and SC external wall. Six of the eight SSSC were at or near CCO and attached to SC external wall at the CCO or in the region of the NSS between ISPC and SC.

 
Conclusions
 

Viscoelastic dilation of SC followed by frontal sectioning permitted exploring distal outflow pathway relationships. ISPC course circumferentially parallel to SC. In 4 eyes/16 segments, parallel ISPC encompassed 50% of SC length. CCO provided perpendicular communications between SC and ISPC. Pressure-dependent TM displacement, through tension on SSSC would also cause displacement of CCO origins and NSS that form one wall of ISPC.

     
Keywords: 427 aqueous • 419 anatomy • 633 outflow: trabecular meshwork  
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