April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Transforming Growth Factor-β2 Effects Expression of Periostin and Procollagen C-endopeptidase Enhancer 1 in Human Trabecular Meshwork Cells
Author Affiliations & Notes
  • Tara Tovar-Vidales
    Cell Biology & Immunology, University of North Texas Hlth Sci Ctr, Fort Worth, TX
    North Texas Eye Research Institute, University of North Texas Hlth Sci Ctr, Fort Worth, TX
  • Monal Naik
    Cell Biology & Immunology, University of North Texas Hlth Sci Ctr, Fort Worth, TX
    North Texas Eye Research Institute, University of North Texas Hlth Sci Ctr, Fort Worth, TX
  • Abbot F Clark
    Cell Biology & Immunology, University of North Texas Hlth Sci Ctr, Fort Worth, TX
    North Texas Eye Research Institute, University of North Texas Hlth Sci Ctr, Fort Worth, TX
  • Robert J Wordinger
    Cell Biology & Immunology, University of North Texas Hlth Sci Ctr, Fort Worth, TX
    North Texas Eye Research Institute, University of North Texas Hlth Sci Ctr, Fort Worth, TX
  • Footnotes
    Commercial Relationships Tara Tovar-Vidales, None; Monal Naik, None; Abbot Clark, Alcon Res, Ltd. (F), Sanofi-FOVEA (C); Robert Wordinger, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5690. doi:
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      Tara Tovar-Vidales, Monal Naik, Abbot F Clark, Robert J Wordinger; Transforming Growth Factor-β2 Effects Expression of Periostin and Procollagen C-endopeptidase Enhancer 1 in Human Trabecular Meshwork Cells. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5690.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Transforming growth beta-2 (TGF-β2) has been associated with increased extracellular matrix (ECM) deposition, which is attributed to increased aqueous humor outflow resistance through the trabecular meshwork (TM). We have previously demonstrated that bone morphogenetic protein 1 (BMP1) (an enzyme responsible for the cleavage and maturation of ECM proteins) is expressed and regulated by TGF-β2 in the human TM and that BMP1 regulates lysyl oxidase activity. Also, other factors associated with the ECM remodeling include periostin (POSTN) and procollagen c-endopeptidase enhancer 1 (PCOLCE1). The purpose of this study was to determine whether human TM cells (a) express POSTN and PCOLCE1 (b) whether expression of POSTN and PCOLCE1 are regulated by TGF-β2.

Methods: Primary human normal (NTM) and glaucomatous (GTM) cells were isolated and subjected to qPCR and Western immunoblotting (WB) for POSTN and PCOLCE1 expression. qPCR was used to determine POSTN and PCOLCE1 expression between control and TGF-β2 treated (5ng/ml for 24 hours) TM cells. WBs of cell lysates and conditioned medium were used to compare POSTN and PCOLCE1 protein expression between control and TGF-β2 treated NTM and GTM cells.

Results: Human TM cells expressed POSTN and PCOLCE1 mRNA and protein. Exogenous TGF-β2 increased POSTN mRNA expression (p<0.05) and decreased PCOLCE1 expression (p<0.0005) compared to control cells. WB analysis showed increased POSTN secretion in NTM compared to GTM cells (p<0.05). TGF-β2 induced POSTN in NTM cells (p<0.05). However, no POSTN was detected in cell lysates of TM cells. WB analysis showed decreased PCOLCE1 secretion in NTM cells compared to GTM cells (p<0.05).

Conclusions: POSTN and PCOLCE1 are expressed in the human TM. These molecules may be involved in the normal function of the TM as well as TM pathogenesis. Altered expression of POSTN and PCOLCE1 may lead to structural and functional changes in the ECM within the TM. Acknowledgement: Supported by NIH: EY017374

Keywords: 735 trabecular meshwork • 543 growth factors/growth factor receptors  
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