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Saori Ohira, Toshihiro Inoue, Hidenobu Tanihara; Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Neovascular Glaucoma. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5698.
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To investigate proinflammatory cytokine levels in the aqueous humor in patients with neovascular glaucoma (NVG), and to analyze the effect of intravitreal bevacizumab injection on the cytokine levels.
This cross-sectional study involved 69 cataract cases, 53 POAG cases and 21 NVG cases. Aqueous humor samples were collected at the beginning of surgery. Bevacizumab was injected into vitreous cavity one or two days prior to the surgery in 14 NVG cases (Group A), while 7 NVG cases received no anti-vascular endothelial growth factor (VEGF) therapy within 3 months before surgery (Group B). Aqueous levels of cytokines were determined by multiplex immunoassay. The data were analyzed using the Tukey-Kramer HSD test.
In Group A, mean (±SD) interleukin (IL)-6, IL-8, monocyte chemotactic protein (MCP)-1, tumor necrosis factor (TNF)-α, platelet-derived growth factor (PDGF)-AA, PDGF-AB/BB, and VEGF levels were 3575.1 ± 4475.7, 483.2 ± 621.4, 3844.4 ± 1370.5, 2.3 ± 0.7, 72.9 ± 48.8, 6.0 ± 9.9 and 0.9 ± 1.9 pg/ml, respectively. In Group B, the corresponding values were 1438.7 ± 2181.6, 82.2 ± 51.9, 2641.3 ± 929.4, 2.2 ± 1.1, 59.7 ± 32.6, 6.2 ± 10.7 and 431.1 ± 566.4 pg/ml, respectively. Of those, the levels of IL-6, IL-8 and MCP-1 were higher in Group A, while the level of VEGF was lower in Group A compared with Group B (P <0.01). There were no significant differences in age, preoperative intraocular pressure, history of ocular surgeries, causative ocular disease of glaucoma, the number of glaucoma eye drops, presence of rubeosis and hyphema between the groups. The levels of all analyzed cytokines except PDGF-AB/BB in Group A are higher than those in cataract cases and POAG cases. The levels of MCP-1 and VEGF in Group B were higher compared with cataract and POAG cases.
The cytokine levels in NVG cases were higher compared with cataract and POAG cases. Intravitreal bevacizumab injection may increase the levels of proinflammatory cytokines in NVG cases.
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