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Wenbin Huang, Shida Chen, Xinbo Gao, Wei Wang, Minwen Zhou, Xiulan Zhang; Multiplex cytokine analysis of aqueous humor in acute primary angle-closure eyes. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5699.
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To measure levels of various inflammation-related cytokines in the aqueous humor of patients with acute primary angle-closure (APAC) and senile cataract.
Aqueous humor samples were prospectively collected from 23 eyes (12 eyes with current APAC, and 11 eyes with previous APAC) of 23 APAC patients and 15 eyes of 15 cataract patients. For the current APAC group, samples were collected during an anterior chamber paracentesis procedure; and for the previous APAC group, samples were collected at the beginning of trabeculectomy. The levels of 15 inflammation-related cytokines in the aqueous humor of APAC and cataract subjects were estimated using the multiplex bead immunoassay technique. Data on patient demographics and preoperative intraocular pressure (IOP) were also collected for correlation analysis.
Compared with the group with previous APAC and the cataract group, the group with current APAC showed clear and significantly elevated concentrations of interleukin (IL)-6, IL-8, granulocyte colony-stimulating factor (G-CSF), monocyte chemotactic protein (MCP)-1, MCP-3 and vascular endothelial growth factor (VEGF) (all p < 0.0167). The group with previous APAC and the cataract group had similar levels of cytokines. IOP was positively correlated with IL-8 (p = 0.001), G-CSF (p = 0.002), MCP-3 (p < 0.001), and VEGF (p < 0.001).
An early ‘‘acute inflammatory’’ condition occurs in eyes with current APAC. In addition to controlling IOP, anti-inflammation treatments are necessary for eyes suffering from APAC.
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