April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Patients with retinopathy of prematurity (ROP) have increased levels of prolactin and reduced levels of vasoinhibins in the circulation
Author Affiliations & Notes
  • Vazquez Membrillo Miguel Angel
    Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico (UNAM), Campus Juriquilla, Mexico
  • Luz Consuelo Zepeda Romero
    OPD, Hospital Civil "Fray Antonio Alcalde", Centro Universitario de Ciencias de la Salud,Universidad de Guadalajara, Guadalajara, Mexico
  • Francisco Gomez Aguayo
    OPD, Hospital Civil "Fray Antonio Alcalde", Centro Universitario de Ciencias de la Salud,Universidad de Guadalajara, Guadalajara, Mexico
  • Jose Alfonso Gutierrez Padilla
    OPD, Hospital Civil "Fray Antonio Alcalde", Centro Universitario de Ciencias de la Salud,Universidad de Guadalajara, Guadalajara, Mexico
  • Eusebio Angulo Castellanos
    OPD, Hospital Civil "Fray Antonio Alcalde", Centro Universitario de Ciencias de la Salud,Universidad de Guadalajara, Guadalajara, Mexico
  • Juan Carlos Barrera de Leon
    Hospital Mateno Infantil "Esperanza López Mateos" Secretaria de Salud Jalisco, Guadalajara, Mexico
  • Cesareo Gonzalez Bernal
    Hospital Mateno Infantil "Esperanza López Mateos" Secretaria de Salud Jalisco, Guadalajara, Mexico
  • Alejandro Quezada Chalita
    OPD, Hospital Civil "Fray Antonio Alcalde", Centro Universitario de Ciencias de la Salud,Universidad de Guadalajara, Guadalajara, Mexico
  • Alonso Meza Anguiano
    OPD, Hospital Civil "Fray Antonio Alcalde", Centro Universitario de Ciencias de la Salud,Universidad de Guadalajara, Guadalajara, Mexico
  • Carmen Clapp
    Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico (UNAM), Campus Juriquilla, Mexico
  • Footnotes
    Commercial Relationships Vazquez Membrillo Miguel Angel, None; Luz Consuelo Zepeda Romero, None; Francisco Gomez Aguayo, None; Jose Alfonso Gutierrez Padilla, None; Eusebio Angulo Castellanos, None; Juan Carlos Barrera de Leon, None; Cesareo Gonzalez Bernal, None; Alejandro Quezada Chalita, None; Alonso Meza Anguiano, None; Carmen Clapp, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5928. doi:
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      Vazquez Membrillo Miguel Angel, Luz Consuelo Zepeda Romero, Francisco Gomez Aguayo, Jose Alfonso Gutierrez Padilla, Eusebio Angulo Castellanos, Juan Carlos Barrera de Leon, Cesareo Gonzalez Bernal, Alejandro Quezada Chalita, Alonso Meza Anguiano, Carmen Clapp; Patients with retinopathy of prematurity (ROP) have increased levels of prolactin and reduced levels of vasoinhibins in the circulation. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5928.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Prolactin (PRL) is proangiogenic but it acquires antiangiogenic properties after undergoing proteolytic cleavage to vasoinhibins, a family of PRL fragments. Given that preterm infants are hyperprolactinemic and that systemic PRL can access ocular tissues to regulate angiogenesis, we hypothesized that reciprocal changes in the levels of systemic PRL and vasoinhibins contribute to the development and progression of ROP. We therefore compared the levels of PRL and vasoinhibins in the circulation of ROP and control premature infants during the first seven weeks after birth.

Methods: Ninety-eight premature patients (birth weight, 500 to 1800 g) matched for gestational age, Apgar score, sepsis, and gender were diagnosed as ROP (n = 53) and control (n = 45) subjects by indirect ophthalmoscopy (at ≥3 weeks postnatal age). PRL was measured by a chemiluminescent immunometric (CI) assay in serum samples obtained every week starting at postnatal week one. Vasoinhibins were evaluated by immunoprecipitation-Western blot and densitometry at postnatal weeks one and three.

Results: Circulating PRL levels were significantly higher in ROP patients than in control patients at week one and at weeks four to seven after birth. These higher levels may reflect the reduced conversion of PRL to vasoinhibins. The CI assay essentially measures PRL, as vasoinhibins have low CI activity (15%). More importantly, the levels of serum vasoinhibins in ROP patients were significantly lower than those in control patients and were undetectable at postnatal weeks one and three, respectively.

Conclusions: These findings show that the ROP condition associates with elevated systemic PRL and its reduced conversion to vasoinhibins. Because PRL and vasoinhibins promote and inhibit angiogenesis, respectively, the reciprocal changes in their levels may represent an efficient mechanism contributing to the development and progression of ROP. Establishing the relation between the levels of PRL and vasoinhibins and infant’s birth weight and severity of ROP will help substantiate these conclusions.

Keywords: 706 retinopathy of prematurity • 700 retinal neovascularization • 638 pathology: human  
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