April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Delivery of the secretagogue Ap4A from conventional and silicone hydrogel soft contact lenses: effect on tear secretion
Author Affiliations & Notes
  • Jesus Pintor
    Bioquimica y Biologia Molecular IV, F. de Optica UCM, Madrid, Spain
  • Carmen Olaya Dominguez-Godinez
    Optica II, F. Optica, UCM, Madrid, Spain
  • Alba Martin-Gil
    Bioquimica y Biologia Molecular IV, F. de Optica UCM, Madrid, Spain
  • Gonzalo Carracedo
    Optica II, F. Optica, UCM, Madrid, Spain
  • Jose Manuel Gonzalez-Meijome
    Clinical & Experimental Optometry Research Lab, Center of Physics, University of Minho, Braga, Portugal
  • Ana Guzman-Aranguez
    Bioquimica y Biologia Molecular IV, F. de Optica UCM, Madrid, Spain
  • Footnotes
    Commercial Relationships Jesus Pintor, None; Carmen Olaya Dominguez-Godinez, None; Alba Martin-Gil, None; Gonzalo Carracedo, None; Jose Manuel Gonzalez-Meijome, None; Ana Guzman-Aranguez, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 6053. doi:
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      Jesus Pintor, Carmen Olaya Dominguez-Godinez, Alba Martin-Gil, Gonzalo Carracedo, Jose Manuel Gonzalez-Meijome, Ana Guzman-Aranguez; Delivery of the secretagogue Ap4A from conventional and silicone hydrogel soft contact lenses: effect on tear secretion. Invest. Ophthalmol. Vis. Sci. 2014;55(13):6053.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate the possible use of soft contact lenses (CL) to improve the secretagogue role of diadenosine tetraphosphate (Ap4A) promoting tear secretion.

Methods: Two conventional hydrogel CL (Omafilcon A and Ocufilcon D) and two silicone hydrogel (SiH) CL (Comfilcon A and Balafilcon A) were used. Ap4A was loaded into the lenses by soaking in a 1 mM Ap4A solution during 12 h. In vitro experiments were performed by placing the lenses in multi-wells during 2 h containing 1 ml of ultrapure water. 100 ul aliquots were taken at time zero and every minute for the first 10 min, and then every 15 min. In vivo experiments were performed in New Zealand rabbits and both the dinucleotide release from SiH and tear secretion were measured by means of Schirmer strips and high-pressure liquid chromatography (HPLC) analysis.

Results: Ap4A in vitro release experiments in hydrogel CL presented a release time 50 (RT50) of 3.9 ± 0.2 min and 3.1 ± 0.1 min for the non-ionic and the ionic CL, respectively. SiH CL released also Ap4A with RT50 values of 5.1 ± 0.1 min for the non-ionic and 2.7 ± 0.1 min for the ionic CL. In vivo experiments with SiH CL showed RT50 values of 9.3 ± 0.2 min and 8.5 ± 0.2 min for the non-ionic and the ionic respectively. The non-ionic lens Ap4A release was able to induce tear secretion above baseline tear levels for almost 360 min.

Conclusions: The delivery of Ap4A is slower and the effect lasts longer with non-ionic lenses than ionic lenses.

Keywords: 486 cornea: tears/tear film/dry eye • 477 contact lens  
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