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Maria Santiago-Varela, Manuel F Bande, Maria José Blanco, Purificacion Mera, Carmela Capeans, Maria Pardo, Antonio Pineiro-Ces; PARK7 DJ1 silenced by siRNA inhibits uveal melanoma cell proliferation. Invest. Ophthalmol. Vis. Sci. 2014;55(13):616.
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DJ-1/PARK7 is an oncoprotein whose aberrant expression has been found in several types of human cancer. Recent studies indicated that DJ-1 is closely related to the proliferation, metastasis, occurrence, and prognosis of the malignant tumors by down-regulating the phosphatase and tensin homologue (PTEN). The overexpression of DJ-1 has been previously described by our group in uveal melanoma (UM) tumors. Additionally, we detected DJ-1 in UM tumor secretomes and in UM patients sera (Pardo et al., Proteomics 2005; Int. J Cancer 2006) Importantly, we have described that high DJ-1 circulating levels are associated with choroidal nevi transformation risk factors (Bande et al., IOVS 2012). However, the pathophysiological role of DJ-1 in UM is still poorly understood. The aim of this work was to investigate the effect of DJ-1inhibition on the proliferation of uveal melanoma established cells lines
Small interfering RNA (siRNA) directed against DJ-1 (Human PARK7siRNAON-TARGETplus SMARTpool, Thermo Scientific Dharmacon) or control (ON-targetplus non-targeting siRNA, Thermo Scientific Dharmacon) was introduced in UM-A, SP6.5, and 92.1 cell lines with Lipofentamine (Invitrogen). Dynamic cell proliferation of silenced DJ-1 vs. non-silenced cells was assayed by real-time monitoring using xCellingence System (ACEA Biosciencies)
Down-regulation of DJ-1expression in UM cells showed a significant decrease of cell proliferation compared to those transfected with control siRNA
DJ-1 oncogene may play a role in the proliferation of uveal melanoma cells in vitro
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