April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Identification of miRNAs associated with herpes simplex keratitis by miRNA microarray analysis
Author Affiliations & Notes
  • Fei Wang
    Eye & ENT hospital, Fudan University, Shanghai, China
  • Tingting Qian
    Eye & ENT hospital, Fudan University, Shanghai, China
  • Xinhan Cui
    Eye & ENT hospital, Fudan University, Shanghai, China
  • JianJiang Xu
    Eye & ENT hospital, Fudan University, Shanghai, China
  • Footnotes
    Commercial Relationships Fei Wang, None; Tingting Qian, None; Xinhan Cui, None; JianJiang Xu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 6285. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Fei Wang, Tingting Qian, Xinhan Cui, JianJiang Xu, ; Identification of miRNAs associated with herpes simplex keratitis by miRNA microarray analysis. Invest. Ophthalmol. Vis. Sci. 2014;55(13):6285.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

To identify the miRNA expression profile of Herpes Simplex Keratitis(HSK) in C57BL/6 mice

 
Methods
 

Mice cornea was infected with herpes simplex virus-1(HSV-1) and then microRNA microarray technique was performed to identify differentially-expressed miRNAs. Some miRNAs were consquently verified by real-time quantitative PCR (qRT-PCR). Functions and locations of theses miRNAs were predicted by databases.

 
Results
 

Upregulation of some microRNAs was identified in HSK cornea including mmu-miR-223-3p, mmu-miR-155-5p, mmu-miR-21-3p, mmu-miR-150-5p, mmu-miR-18a-5p, mmu-miR-3096b-5p, mmu-miR-146a-5p and mmu-miR-342-3p. While, downregulation of mmu-miR-434-3p, mmu-miR-124-3p, mmu-miR-204-5p, mmu-miR-133b-3p, mmu-miR-206-3p and mmu-miR-133a-3p was found in HSK. Moreover, the differential expression of mmu-miR-204-5p, mmu-miR-206-3p, mmu-miR-155-5p, mmu-miR-133a-3p and mmu-miR-150-5p were identified by qRT-PCR in HSK cornea tissues. Their putative target genes were predicted using 2 online software programs

 
Conclusions
 

Some new miRNAs are found in HSK, which might play significant roles in regulating in pathological process.

 
 
Clustering image including all 34 differentially expressed miRNAs.
 
Clustering image including all 34 differentially expressed miRNAs.
 
 
Histograms of miRNAs based on QRT-PCR results.
 
Histograms of miRNAs based on QRT-PCR results.
 
Keywords: 480 cornea: basic science  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×