April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
The Effect of Titanium Dioxide Nanoparticles on the Developing Retina
Author Affiliations & Notes
  • Yuhao Li
    Department of Pathology, Nankai University School of Medicine, Tianjin, China
  • Yajie Wang
    Department of Pathology, Nankai University School of Medicine, Tianjin, China
  • Zizi He
    Department of Pathology, Nankai University School of Medicine, Tianjin, China
  • Yangwu Fang
    Department of Pathology, Nankai University School of Medicine, Tianjin, China
  • Yang Xu
    State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin, China
  • Yanan Chen
    Department of Pathology, Nankai University School of Medicine, Tianjin, China
  • Footnotes
    Commercial Relationships Yuhao Li, None; Yajie Wang, None; Zizi He, None; Yangwu Fang, None; Yang Xu, None; Yanan Chen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 701. doi:
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    • Get Citation

      Yuhao Li, Yajie Wang, Zizi He, Yangwu Fang, Yang Xu, Yanan Chen; The Effect of Titanium Dioxide Nanoparticles on the Developing Retina. Invest. Ophthalmol. Vis. Sci. 2014;55(13):701.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To investigate whether TiO2 NPs was toxic on zebrafish embryos and developing retina via aqueous exposure.

 
Methods
 

Embryonic zebrafish were exposed to TiO2 NPs at a concentration of 1mg/L from 1-4 cell stage to 72 hpf. The mortality and hatching rate were calculated at 24 hpf and 48 hpf respcetively. An mRNA probe for atonal homolog 7 (atoh7) was used as a marker to explore whether the neurogenesis initiated on time. Differentiation of ganglion cells, cones and rods was determined using immunohistochemistry at 72hpf with Zn12, Zpr1 and Zpr3 antibodies respectively. Microglia in the brain and retina were labeled using an mRNA probe for fms.

 
Results
 

TiO2 NPs exposure did not induce any embryonic developmental malformations. The onset of neurogenesis and neuronal differentiation were not delayed following TiO2 exposure. The location and number of microglia didn’t have a significant difference between the TiO2 NPs exposed and control group.

 
Conclusions
 

TiO2 NPs exposure at 1mg/L doesn’t disrupt the development of zebrafish embryos and retinal neurogenesis.

     
Keywords: 608 nanomedicine • 698 retinal development  
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