April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
In vivo Visualization of Posterior Lamina Cribrosa (LC) using SD-OCT Validated with Histology
Author Affiliations & Notes
  • HUONG TRAN
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Bo Wang
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Ning-Jiun Jan
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Gadi Wollstein
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Matthew A Smith
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Larry Kagemann
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Hiroshi Ishikawa
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Joel S Schuman
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Elizabeth Tyler-Kabara
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    Neurological Surgery, University of Pittsburgh, Pittsburgh, PA
  • Ian A Sigal
    Bioengineering, University of Pittsburgh, Pittsburgh, PA
    UPMC Eye Ctr/Eye and Ear Inst/Ophthal, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • Footnotes
    Commercial Relationships HUONG TRAN, None; Bo Wang, None; Ning-Jiun Jan, None; Gadi Wollstein, None; Matthew Smith, None; Larry Kagemann, None; Hiroshi Ishikawa, None; Joel Schuman, Carl Zeiss Meditec (P); Elizabeth Tyler-Kabara, None; Ian Sigal, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 913. doi:
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      HUONG TRAN, Bo Wang, Ning-Jiun Jan, Gadi Wollstein, Matthew A Smith, Larry Kagemann, Hiroshi Ishikawa, Joel S Schuman, Elizabeth Tyler-Kabara, Ian A Sigal; In vivo Visualization of Posterior Lamina Cribrosa (LC) using SD-OCT Validated with Histology. Invest. Ophthalmol. Vis. Sci. 2014;55(13):913.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The LC plays a central role in the pathophysiology of glaucoma, and therefore its visualization has great potential to help understand the disease. Spectral domain (SD-) OCT has been demonstrated suitable to visualize the anterior surface of the LC in vivo, but the visibility of the posterior LC remains controversial. Our goal was to test the hypothesis that SD-OCT allows visualization of the posterior LC consistently across scans and eyes, and with sufficient quality to discern the microstructure of even the most posterior LC.

 
Methods
 

Eyes from two monkeys were cannulated and IOP was controlled using gravity perfusion. One eye from each monkey was scanned three times using OCT (Bioptigen, 3mm x 3mm, 512 x 512 pixels). Following this, both eyes were enucleated and perfusion fixed at an IOP of 30mmHg, 30 minutes post-death. They were then cryosectioned and imaged with a stereomicroscope (Nikon SMZ1500, 16bit greyscale, 0.765μm/pixel). The OCT-scanned eyes were sectioned coronally to verify the OCT-histology correspondence of LC microstructure. The non-OCT-scanned eyes were sectioned sagittally to evaluate the penetration depth of OCT signal. We delineated the visible posterior LC every 8th B-scan and computed the fraction of the scleral canal (defined by Bruch’s membrane opening - BMO). We performed the delineation in the three scans from each monkey, and the results compared to determine the repeatability of LC visibility.

 
Results
 

Histology confirmed that the most posterior LC was visible in the OCT images (Figure). Qualitative comparison shows correspondence in LC microstructure between OCT and histology (Figure). Quantitative analysis shows comparable LC thickness: 335±4µm for OCT and 330±30µm for histology. Using BMO plane as a reference, the fraction of visible posterior LC was approximately 50% and was consistent within the three OCT scans from each monkey (Table).

 
Conclusions
 

SD-OCT allows visualization of the entire monkey LC thickness in vivo including the most posterior pores and beams. Posterior LC was consistently identified in approximately half of the area of the scleral canal defined by BMO. The extent to which our results apply to humans remains to be determined.

 
 
Figure: Comparison of penetration depth and LC microstructure between OCT (top row) and histology (bottom row). Yellow lines illustrate corresponding structures.
 
Figure: Comparison of penetration depth and LC microstructure between OCT (top row) and histology (bottom row). Yellow lines illustrate corresponding structures.
 
 
Table: Results of delineating posterior LC in the OCT scans.
 
Table: Results of delineating posterior LC in the OCT scans.
 
Keywords: 577 lamina cribrosa • 551 imaging/image analysis: non-clinical • 599 microscopy: light/fluorescence/immunohistochemistry  
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