June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
INNOVATIVE USE OF SPECTRALIS OCT AUTOFLUORESCENCE ON TRACKING EX-VIVO HUMAN CORNEAL ENDOTHELIAL CELL COLONIES.
Author Affiliations & Notes
  • Gustavo T Grottone
    Ophthalmology, UNIFESP/Santa Casa de Santos, Santos, Brazil
  • Jose Alvaro Pereira Gomes
    Ophthalmology, UNIFESP/Santa Casa de Santos, Santos, Brazil
  • Joyce Luciana Covre
    Ophthalmology, UNIFESP/Santa Casa de Santos, Santos, Brazil
  • Renata Ruoco Loureiro
    Ophthalmology, UNIFESP/Santa Casa de Santos, Santos, Brazil
  • Nicolas C Pereira
    Ophthalmology, UNIFESP/Santa Casa de Santos, Santos, Brazil
  • Footnotes
    Commercial Relationships Gustavo Grottone, None; Jose Gomes, None; Joyce Covre, None; Renata Loureiro, None; Nicolas Pereira, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 1155. doi:
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      Gustavo T Grottone, Jose Alvaro Pereira Gomes, Joyce Luciana Covre, Renata Ruoco Loureiro, Nicolas C Pereira; INNOVATIVE USE OF SPECTRALIS OCT AUTOFLUORESCENCE ON TRACKING EX-VIVO HUMAN CORNEAL ENDOTHELIAL CELL COLONIES.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1155.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

DEVELOP A NEW METHOD TO TRACK ADHESION AND MIGRATION PATTERNS OF HUMAN CORNEAL ENDOTHELIAL CELLS AFTER EX-VIVO IMPLANTATION.

 
Methods
 

Human corneal endothelial cells previously cultured in vitro were divided in two groups: exposed or non-exposed to magnetic nanoparticles. Those cells were used as an attempt to regenerate our ex-vivo host corneas. Cell colonies were tracked after 7 days using an scanning laser ophthalmoscope with a excitation light of 488 nm. Human corneal endothelial cells previously cultured in vitro were divided in two groups: exposed or non-exposed to magnetic nanoparticles. Those cells were used as an attempt to regenerate our ex-vivo host corneas. Cell colonies were tracked after 7 days using an scanning laser ophthalmoscope with a excitation light of 488 nm.

 
Results
 

Human corneal endothelial cells showed no toxicity when exposed to the studied nanoparticle(p=0.507). Both, non-magnetic and magnetic cells were found attached to posterior surface of corneas after 7 days of ex-vivo cultures and visualized by C-SLO Spectralis autofluorescence mode.

 
Conclusions
 

Human corneal endothelial cells from magnetic and non-magnetic groups attached to corneal posterior surface on ex-vivo hosts.  

 
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