June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Long-term increase of TGF-β1, TGF-β2 and MCP-1 in the aqueous humor of pseudophakic Fuchs Dystrophy Eyes
Author Affiliations & Notes
  • Mario Matthaei
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Johannes Gillessen
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Philipp S Muether
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Robert Hoerster
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Bjoern O Bachmann
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Arno Hueber
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Claus Cursiefen
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Ludwig M Heindl
    Dept. of Ophthalmology, University of Cologne, Cologne, Germany
  • Footnotes
    Commercial Relationships Mario Matthaei, None; Johannes Gillessen, None; Philipp Muether, None; Robert Hoerster, None; Bjoern Bachmann, None; Arno Hueber, None; Claus Cursiefen, None; Ludwig Heindl, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 1170. doi:
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      Mario Matthaei, Johannes Gillessen, Philipp S Muether, Robert Hoerster, Bjoern O Bachmann, Arno Hueber, Claus Cursiefen, Ludwig M Heindl; Long-term increase of TGF-β1, TGF-β2 and MCP-1 in the aqueous humor of pseudophakic Fuchs Dystrophy Eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1170.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To analyze the specific expression of distinct epithelial-mesenchymal transition (EMT)-associated proteins in the aqueous humor of phakic and pseudophakic FECD eyes.

Methods: Fifty aqueous humor samples from phakic FECD eyes (FECDph), from pseudophakic FECD eyes with more than one year post-cataract surgery (FECDpsph) and from cataract controls without FECD (Controlcat) were collected during Descemet membrane endothelial keratoplasty (DMEK) or cataract surgery. The expression of EMT-associated proteins (TGF-β1, TGF-β2, TGF-β3, MCP-1, FGF-basic, TNF-α, Il-1ß) was determined by multiplex bead assay and comparatively analyzed.

Results: FECDpsph eyes showed significantly elevated expression compared to FECDph and Controlcat groups respectively for TGF-β1 (p=0.0011 and p<0.0001), TGF-β2 (p=0.0288 and p=0.0076) and MCP-1 (p=0.0007 and p<0.0001). No differences in EMT-associated protein levels were detected comparing FECDph eyes and Controlcat eyes.

Conclusions: Cataract surgery causes long-term elevated levels of distinct EMT-associated proteins (TGF-β1, TGF-β2, MCP-1) in the aqueous humor of FECD eyes. This may contribute to accelerated FECD progression post cataract surgery. No alterations in EMT-associated protein levels were found in phakic FECD eyes.

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