June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Epithelial Wound Healing and Heat shock protein 27 phosphorylation in NaCl-induced hyperosmolar condition
Author Affiliations & Notes
  • Jae Yong Kim
    Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (the Republic of)
  • Soon-Suk Kang
    Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (the Republic of)
  • Eun Soon Kim
    Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (the Republic of)
  • Myoung Joon Kim
    Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (the Republic of)
  • Hungwon Tchah
    Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships Jae Yong Kim, None; Soon-Suk Kang, None; Eun Soon Kim, None; Myoung Joon Kim, None; Hungwon Tchah, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 1191. doi:
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      Jae Yong Kim, Soon-Suk Kang, Eun Soon Kim, Myoung Joon Kim, Hungwon Tchah; Epithelial Wound Healing and Heat shock protein 27 phosphorylation in NaCl-induced hyperosmolar condition. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1191.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We attempt to investigate the epithelial wound healing and heat shock protein 27 (HSP27) phoshorylation in hyperosmolar condition.

Methods: Telomerase-immortalized human corneal epithelial cells (hTCEpi) were cultured in NaCl-induced hyperosmolar condition. After scratch-induced directional wounding was created, epithelial migration was evaluated. Western blotting was performed using antibodies against phosphorylated and non-phosphorylated HSP27.

Results: The epithelial wound healing was attenuated in the NaCl-induced hyperosmolar condition. Contrary to the non-phosphorylated HSP27, the phosphorylation of HSP27 increased in the NaCl-induced hyperosmolar condition. The higher hyperosmolar condition, the more wound healing was attenuated and the more HSP27 phosphorylation was induced.

Conclusions: The HSP27 phosphorylation can be induced and play a role in epithelial migration in the dry eye-related hyperosmolar condition.

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