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Jae Yong Kim, Soon-Suk Kang, Eun Soon Kim, Myoung Joon Kim, Hungwon Tchah; Epithelial Wound Healing and Heat shock protein 27 phosphorylation in NaCl-induced hyperosmolar condition. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1191.
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© ARVO (1962-2015); The Authors (2016-present)
We attempt to investigate the epithelial wound healing and heat shock protein 27 (HSP27) phoshorylation in hyperosmolar condition.
Telomerase-immortalized human corneal epithelial cells (hTCEpi) were cultured in NaCl-induced hyperosmolar condition. After scratch-induced directional wounding was created, epithelial migration was evaluated. Western blotting was performed using antibodies against phosphorylated and non-phosphorylated HSP27.
The epithelial wound healing was attenuated in the NaCl-induced hyperosmolar condition. Contrary to the non-phosphorylated HSP27, the phosphorylation of HSP27 increased in the NaCl-induced hyperosmolar condition. The higher hyperosmolar condition, the more wound healing was attenuated and the more HSP27 phosphorylation was induced.
The HSP27 phosphorylation can be induced and play a role in epithelial migration in the dry eye-related hyperosmolar condition.
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