June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
The effect of anterior chamber pressure and corneal drying on corneal thickness in preparation for descemet stripping endothelial keratoplasty (DSAEK).
Author Affiliations & Notes
  • Bernhard Steger
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Vito Romano
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Sherif Hassaan
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Mark Batterbury
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Sajjad Ahmad
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Colin E Willoughby
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Ahmed Elsheikh
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Jern Yee Chen
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Stephen B Kaye
    Department of Corneal and External Eye Diseases, St. Paul's Eye Unit, Royal Liverpool University Hospital, University of Liverpool, Liverpool, United Kingdom
  • Footnotes
    Commercial Relationships Bernhard Steger, None; Vito Romano, None; Sherif Hassaan, None; Mark Batterbury, None; Sajjad Ahmad, None; Colin Willoughby, None; Ahmed Elsheikh, None; Jern Chen, None; Stephen Kaye, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 1568. doi:
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      Bernhard Steger, Vito Romano, Sherif Hassaan, Mark Batterbury, Sajjad Ahmad, Colin E Willoughby, Ahmed Elsheikh, Jern Yee Chen, Stephen B Kaye; The effect of anterior chamber pressure and corneal drying on corneal thickness in preparation for descemet stripping endothelial keratoplasty (DSAEK).. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1568.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To investigate the effect of anterior chamber pressure and corneal drying on graft thickness in graft preparation for DSAEK.

 
Methods
 

Corneoscleral discs were placed in an artificial anterior chamber (AAC). AAC pressure (15mmHg, 45mmHg, 92mmHg, 109mmHg and 198mmHg) was controlled using the height of an infusion bottle and a clamp. Corneas were exposed or repeatedly dried at 1minute intervals using cellulose spears. The endothelium was removed in one group. 3 corneas were used for each condition. Central corneal thickness was measured independently by two observers every 90 seconds for the first 15 minutes and every 5 minutes for the next 10 minutes using an ultrasound pachymeter (SP-100, Tomey).

 
Results
 

There was a significant inverse linear relationship between corneal thickness, AAC pressure and corneal drying (Table). . Removal of corneal endothelium reduced the rate of corneal thinning.

 
Conclusions
 

Increasing AAC pressure and corneal drying reduce graft thickness at predictable rates. For higher pressures and drying, the rate of thinning lay between narrow 95% confidence intervals, providing a reliable method to reduce corneal thickness in the preparation of grafts for DSAEK.  

 
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