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Masaru Takeuchi, Atsushi Tanaka, Kouzo Harimoto, Izumi Mine, Tomohito Sato, Yoko Karasawa, Takayuki Kanda; Analysis of Th cell-related cytokine production in Behçet’s disease patients with uveitis before and after treatment with infliximab. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1718.
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© ARVO (1962-2015); The Authors (2016-present)
Infliximab (Inflix), is an anti- TNFα monoclonal antibody, resolves effectively ocular inflammation in Behçet's disease (BD) patients by suppressing inflammatory process via TNFα. It is reported that treatment with Inflix inhibits Th-cell differentiation into Th17 cells and promotes regulatory T cell production in BD patients with uveitis.<br /> In this study, we examined quantitative changes of cytokines produced by various Th cells specific for ocular tissue antigens before and after treatment with Inflix in BD patients with uveitis.
Eight BD patients with uveitis during treatment with infliximab were recruited in this study. Peripheral blood mononuclear cells (PBMCs) were obtained before infusion of infliximab and at 1 week after the infusion. PBMCs were also obtained from 10 healthy subjects as the control. Each sample was cultured for 48 hrs with IRBP, and supernatants were collected at the end of culture. IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-25, IL-31, IL-33, IFN-γ, sCD40L, and TNFα in the supernatants were measured by Bio-Plex kit®. The study was approved by the institutional review board of National Defense Medical College.
All measured cytokines except for IL-25 and IL-33 were higher in BD patients before Inflix infusion compared with BD patients after Inflix infusion or healthy subjects. The levels of IL-4, IL-10, and sCD40L in BD patients after Inflix infusion were comparable with those of healthy subjects, however IL-1β, IL-6, IL-17A, IL-17F, IL-21, IL-22, IL-31, IFN-γ, and TNFα were still higher in BD patients than in healthy subjects.
It was indicated that cytokines produced by Th cells stimulated with IRBP in BD patients were suppressed by Inflix treatment, which was not only cytokines promoting ocular inflammation but also the inhibitory cytokines, but that the levels of most cytokines did not reach to those of healthy subjects.
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